Exploring the versatility of pentafulvene-maleimide cycloaddition as a ligation strategy: buffer and pH effects.

Cycloaddition Reaction Maleimides / chemistry Hydrolysis Kinetics Hydrogen-Ion Concentration Buffers

Journal

Organic & biomolecular chemistry
ISSN: 1477-0539
Titre abrégé: Org Biomol Chem
Pays: England
ID NLM: 101154995

Informations de publication

Date de publication:
09 Nov 2022
Historique:
pubmed: 25 10 2022
medline: 11 11 2022
entrez: 24 10 2022
Statut: epublish

Résumé

Ligation chemistries are often required to perform under stringent conditions that preserve the integrity and function of (bio)conjugates, including specific biological buffers. To explore the versatility of the pentafulvene-maleimide ligation for (bio)conjugations, we studied the stability of the coupling partners and their Diels-Alder cycloaddition (DAC) in buffers used commonly in biological assays, protein chemistry and bioconjugates. The stability of 6,6-dialkylpentafulvene and maleimide derivatives to a panel of buffers with pH values between 3.7 and 10.1 was monitored

Identifiants

DOI: 10.1039/d2ob01453j PMID: 36278771
pubmed: 36278771
doi: 10.1039/d2ob01453j
doi:

Substances chimiques

maleimide 2519R1UGP8
Maleimides 0
Buffers 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

8538-8544

Auteurs

Kirsten Platts (K)

Applied Chemistry and Translational Biomaterials Group, Clinical and Health Sciences, University of South Australia, Adelaide, South Australia, 5000, Australia. anton.blencowe@unisa.edu.au.

Manisha Hota (M)

Applied Chemistry and Translational Biomaterials Group, Clinical and Health Sciences, University of South Australia, Adelaide, South Australia, 5000, Australia. anton.blencowe@unisa.edu.au.
ORCID: 0000-0002-2131-5568

Chris Blencowe (C)

Fleet Bioprocessing, Hartley Whitney, Hampshire, RG27 8DH, UK.

Anton Blencowe (A)

Applied Chemistry and Translational Biomaterials Group, Clinical and Health Sciences, University of South Australia, Adelaide, South Australia, 5000, Australia. anton.blencowe@unisa.edu.au.
ORCID: 0000-0002-7630-4874

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Réaction de cycloaddition › Exploring the versatility of...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Azoles › Pyrroles › Maléimides › Exploring the versatility of...
questionsmedicales.fr › Phénomènes chimiques › Hydrolyse › Exploring the versatility of...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Exploring the versatility of...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Exploring the versatility of...
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