A de novo gene originating from the mitochondria controls floral transition in Arabidopsis thaliana.
Arabidopsis
De novo gene
Flowering
Intergenomic Gene Transfer
Journal
Plant molecular biology
ISSN: 1573-5028
Titre abrégé: Plant Mol Biol
Pays: Netherlands
ID NLM: 9106343
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
received:
05
09
2022
accepted:
09
10
2022
pubmed:
29
10
2022
medline:
21
1
2023
entrez:
28
10
2022
Statut:
ppublish
Résumé
De novo genes created in the plant mitochondrial genome have frequently been transferred into the nuclear genome via intergenomic gene transfer events. Therefore, plant mitochondria might be a source of de novo genes in the nuclear genome. However, the functions of de novo genes originating from mitochondria and the evolutionary fate remain unclear. Here, we revealed that an Arabidopsis thaliana specific small coding gene derived from the mitochondrial genome regulates floral transition. We previously identified 49 candidate de novo genes that induce abnormal morphological changes on overexpression. We focused on a candidate gene derived from the mitochondrial genome (sORF2146) that encodes 66 amino acids. Comparative genomic analyses indicated that the mitochondrial sORF2146 emerged in the Brassica lineage as a de novo gene. The nuclear sORF2146 emerged following an intergenomic gene transfer event in the A. thaliana after the divergence between Arabidopsis and Capsella. Although the nuclear and mitochondrial sORF2146 sequences are the same in A. thaliana, only the nuclear sORF2146 is transcribed. The nuclear sORF2146 product is localized in mitochondria, which may be associated with the pseudogenization of the mitochondrial sORF2146. To functionally characterize the nuclear sORF2146, we performed a transcriptomic analysis of transgenic plants overexpressing the nuclear sORF2146. Flowering transition-related genes were highly regulated in the transgenic plants. Subsequent phenotypic analyses demonstrated that the overexpression and knockdown of sORF2146 in transgenic plants resulted in delayed and early flowering, respectively. These findings suggest that a lineage-specific de novo gene derived from mitochondria has an important regulatory effect on floral transition.
Identifiants
pubmed: 36306001
doi: 10.1007/s11103-022-01320-6
pii: 10.1007/s11103-022-01320-6
doi:
Substances chimiques
Arabidopsis Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
189-203Subventions
Organisme : Grants-in-Aid for Scientific Research
ID : 21H05724
Organisme : Grants-in-Aid for Scientific Research
ID : 20H03317
Organisme : Grants-in-Aid for Scientific Research
ID : 20H05905
Organisme : Grants-in-Aid for Scientific Research
ID : 25710017
Organisme : Grants-in-Aid for Scientific Research
ID : 18KK0176
Organisme : Grants-in-Aid for Scientific Research
ID : 19H05348
Organisme : Grants-in-Aid for Scientific Research
ID : 18H02420
Organisme : Grants-in-Aid for Scientific Research
ID : 19K22313
Organisme : Grants-in-Aid for Scientific Research
ID : 22K14870
Organisme : Grants-in-Aid for Scientific Research
ID : 22H05731
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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