Head-to-tail polymerization by VEL proteins underpins cold-induced Polycomb silencing in flowering control.
CP: Molecular biology
CP: Plants
DNAJ co-chaperones
Polycomb silencing
atypical four-helix bundle
biomolecular condensation
cold-induced flowering control
head-to-tail polymerization
inheritance of repressed state
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
08 11 2022
08 11 2022
Historique:
received:
23
06
2022
revised:
30
08
2022
accepted:
14
10
2022
entrez:
9
11
2022
pubmed:
10
11
2022
medline:
15
11
2022
Statut:
ppublish
Résumé
Transcriptional silencing through the Polycomb silencing machinery utilizes a "read-write" mechanism involving histone tail modifications. However, nucleation of silencing and long-term stable transmission of the silenced state also requires P-olycomb Repressive Complex 2 (PRC2) accessory proteins, whose molecular role is poorly understood. The Arabidopsis VEL proteins are accessory proteins that interact with PRC2 to nucleate and propagate silencing at the FLOWERING LOCUS C (FLC) locus, enabling early flowering in spring. Here, we report that VEL proteins contain a domain related to an atypical four-helix bundle that engages in spontaneous concentration-dependent head-to-tail polymerization to assemble dynamic biomolecular condensates. Mutations blocking polymerization of this VEL domain prevent Polycomb silencing at FLC. Plant VEL proteins thus facilitate assembly of dynamic multivalent Polycomb complexes required for inheritance of the silenced state.
Identifiants
pubmed: 36351412
pii: S2211-1247(22)01476-0
doi: 10.1016/j.celrep.2022.111607
pmc: PMC7614096
mid: EMS160049
pii:
doi:
Substances chimiques
MADS Domain Proteins
0
Arabidopsis Proteins
0
Polycomb-Group Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
111607Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M011216/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/J/000CA378
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/J/00000581
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/J/000PR9773
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/G01406X/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/J/0000A219
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/C517633/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/J/000CA376
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/J/000CA369
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : G20334
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_U105192713
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/G009562/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/J/000CA355
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/J/000CA344
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/J/000PR9788
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_U105184326
Pays : United Kingdom
Organisme : Cancer Research UK
ID : C7379/A24639
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/J/0000A199
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/E009662/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/D010799/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : U105192713
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/J/000CA305
Pays : United Kingdom
Informations de copyright
Crown Copyright © 2022. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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