Quantification of Protein Palmitoylation by Cysteine-SILAC.
Cysteine-SILAC
Mass spectrometry
Metabolic labeling
Protein palmitoylation
Selective enrichment
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
entrez:
12
11
2022
pubmed:
13
11
2022
medline:
16
11
2022
Statut:
ppublish
Résumé
Cysteine-SILAC enables the detection and quantification of protein S-palmitoylation, an important protein posttranslational modification. Here we describe the cell culture, protein extraction, selective enrichment, mass spectrometry, and data analysis for palmitoylated proteins from cell samples by this method.
Identifiants
pubmed: 36370270
doi: 10.1007/978-1-0716-2863-8_5
doi:
Substances chimiques
Cysteine
K848JZ4886
Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
59-69Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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