Synthesis of 5'-fluorophosphate-modified short-interfering RNAs.

RNA, Small Interfering Organophosphorus Compounds Fluorides RNA Interference
Phosphofluoridate RNA nucleic acid chemistry solid phase synthesis

Journal

Nucleosides, nucleotides & nucleic acids
ISSN: 1532-2335
Titre abrégé: Nucleosides Nucleotides Nucleic Acids
Pays: United States
ID NLM: 100892832

Informations de publication

Date de publication:
2023
Historique:
medline: 29 3 2023
pubmed: 22 11 2022
entrez: 21 11 2022
Statut: ppublish

Résumé

We have developed an improved scheme for the synthesis of a mono-fluorinated phosphoramidite for the 5'-modification of nucleic acids using standard phosphoramidite chemistry. We describe the first report of a phosphofluoridate modified siRNA strand and have evaluated C18 HPLC for purification of modified strands from unreacted siRNA strands. Lastly, the biological activity of the high purity siRNA strands, when placed on the sense and/or antisense strand, was evaluated to assess the impact of 5' phosphofluoridate modifications on siRNA activity.

Identifiants

DOI: 10.1080/15257770.2022.2148693 PMID: 36408961
pubmed: 36408961
doi: 10.1080/15257770.2022.2148693
doi:

Substances chimiques

RNA, Small Interfering 0
phosphoramidite 0
fluorophosphate 15181-43-8
Organophosphorus Compounds 0
Fluorides Q80VPU408O

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

358-370

Auteurs

Andrew Varley (A)

Faculty of Science, University of Ontario Institute of Technology, Oshawa, Canada.

Jean-Paul Desaulniers (JP)

Faculty of Science, University of Ontario Institute of Technology, Oshawa, Canada.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › Petit ARN interférent › Synthesis of 5'-fluorophosphate-modified...
questionsmedicales.fr › Composés chimiques organiques › Composés organiques du phosphore › Synthesis of 5'-fluorophosphate-modified...
questionsmedicales.fr › Produits chimiques inorganiques › Composés du fluor › Acide fluorhydrique › Fluorures › Synthesis of 5'-fluorophosphate-modified...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Interférence par ARN › Synthesis of 5'-fluorophosphate-modified...