Rad52's DNA annealing activity drives template switching associated with restarted DNA replication.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 11 2022
26 11 2022
Historique:
received:
10
05
2022
accepted:
17
11
2022
entrez:
26
11
2022
pubmed:
27
11
2022
medline:
30
11
2022
Statut:
epublish
Résumé
It is thought that many of the simple and complex genomic rearrangements associated with congenital diseases and cancers stem from mistakes made during the restart of collapsed replication forks by recombination enzymes. It is hypothesised that this recombination-mediated restart process transitions from a relatively accurate initiation phase to a less accurate elongation phase characterised by extensive template switching between homologous, homeologous and microhomologous DNA sequences. Using an experimental system in fission yeast, where fork collapse is triggered by a site-specific replication barrier, we show that ectopic recombination, associated with the initiation of recombination-dependent replication (RDR), is driven mainly by the Rad51 recombinase, whereas template switching, during the elongation phase of RDR, relies more on DNA annealing by Rad52. This finding provides both evidence and a mechanistic basis for the transition hypothesis.
Identifiants
pubmed: 36435847
doi: 10.1038/s41467-022-35060-4
pii: 10.1038/s41467-022-35060-4
pmc: PMC9701231
doi:
Substances chimiques
DNA
9007-49-2
Rad51 Recombinase
EC 2.7.7.-
Schizosaccharomyces pombe Proteins
0
rad52 protein, S pombe
0
DNA-Binding Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7293Subventions
Organisme : Medical Research Council
ID : MR/V009214/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 090767/Z/09/Z
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P019706/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P028292/1
Pays : United Kingdom
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2022. The Author(s).
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