Differential gene expression associated with flower development of mango (Mangifera indica L.) varieties with different shelf-life.
Differential gene expression
Mango
RNA sequencing
Shelf-life
Transcriptome
Journal
Gene expression patterns : GEP
ISSN: 1872-7298
Titre abrégé: Gene Expr Patterns
Pays: Netherlands
ID NLM: 101167473
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
received:
15
06
2022
revised:
22
11
2022
accepted:
09
12
2022
pubmed:
17
12
2022
medline:
15
3
2023
entrez:
16
12
2022
Statut:
ppublish
Résumé
Mango (Mangifera indica L.) is one of the most important commercial fruit crop grown in many parts of the world. Major challenges affecting mango trade are short shelf-life, high susceptibility to chilling injury, post-harvest diseases and consumer demand for improved fruit quality. The objective of the present study was to reveal the key regulators present in bud and flower tissues during flower development stage, associated with fruit development and affect the shelf-life of the mango fruit. RNA-sequencing of contrasting genotypes having short and long shelf-life, was carried out. Comparative differential expression pathway studies of long shelf-life (Totapuri) and short shelf-life (Bombay Green) mango genotypes revealed a total of 177 highly differentially expressed genes. Out of 177 total genes, 101 genes from endoplasmic reticulum pathway and very few from gibberellins (3) and jasmonic acid (1) pathway were identified. Genes from endoplasmic reticulum pathway like hsp 90, SRC2, DFRA, CHS, BG3 and ASPG1 mainly up regulated in Bombay Green. Uniprotein B9R8D3 also shows up regulation in Bombay Green. Ethylene insensitive pathway gene EIL1 up regulated in Bombay Green. Gene CAD1 from phenylpropanoid pathway mainly up regulated in Bombay Green. A total of 4 SSRs and 227 SNPs were mined from these pathways specific to the shelf-life. Molecular studies of endoplasmic reticulum, phenylpropanoid, ethylene, polygalacturonase and hormone pathways at the time of bud and flower formation revealed key regulators that determine the shelf-life of mango fruit.
Identifiants
pubmed: 36526239
pii: S1567-133X(22)00071-0
doi: 10.1016/j.gep.2022.119301
pii:
doi:
Substances chimiques
Ethylenes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
119301Informations de copyright
Copyright © 2022 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no conflict interest.