Demographic and disease-related factors impact on cerebrospinal fluid neurofilament light chain levels in multiple sclerosis.

biomarker brain atrophy disease-modifying therapies multiple sclerosis neurofilament light chain progressive multiple sclerosis relapsing-remitting multiple sclerosis

Journal

Brain and behavior
ISSN: 2162-3279
Titre abrégé: Brain Behav
Pays: United States
ID NLM: 101570837

Informations de publication

Date de publication:
01 2023
Historique:
revised: 07 11 2022
received: 21 09 2022
accepted: 13 12 2022
pubmed: 28 12 2022
medline: 21 1 2023
entrez: 27 12 2022
Statut: ppublish

Résumé

Neurofilament light (NfL) levels reflect inflammatory disease activity in multiple sclerosis (MS), but it is less clear if NfL also can serve as a biomarker for MS progression in treated patients without relapses and focal lesion accrual. In addition, it has not been well established if clinically effective treatment re-establishes an age and sex pattern for cerebrospinal fluid NfL (cNfL) as seen in controls, and to what degree levels are affected by disability level and magnetic resonance imaging (MRI) atrophy metrics. We included subjects for whom cNfL levels had been determined as per clinical routine or in clinical research, classified as healthy controls (HCs, n = 89), MS-free disease controls (DCs, n = 251), untreated MS patients (uMS; n = 296), relapse-free treated MS patients (tMS; n = 78), and ProTEct-MS clinical trial participants (pMS; n = 41). Using linear regression, we found a positive association between cNfL and age, as well as lower concentrations among women, in all groups, except for uMS patients. In contrast, disability level in the entire MS cohort, or T1 and T2 lesion volumes, brain parenchymal fraction, thalamic fraction, and cortical thickness in the pMS trial cohort, did not correlate with cNfL concentrations. Furthermore, the cNfL levels in tMS and pMS groups did not differ. In participants with MS lacking signs of inflammatory disease activity, disease modulatory therapy reinstates an age and sex cNfL pattern similar to that of control subjects. No significant association was found between cNfL levels and clinical worsening, disability level, or MRI metrics.

Sections du résumé

BACKGROUND
Neurofilament light (NfL) levels reflect inflammatory disease activity in multiple sclerosis (MS), but it is less clear if NfL also can serve as a biomarker for MS progression in treated patients without relapses and focal lesion accrual. In addition, it has not been well established if clinically effective treatment re-establishes an age and sex pattern for cerebrospinal fluid NfL (cNfL) as seen in controls, and to what degree levels are affected by disability level and magnetic resonance imaging (MRI) atrophy metrics.
METHODS
We included subjects for whom cNfL levels had been determined as per clinical routine or in clinical research, classified as healthy controls (HCs, n = 89), MS-free disease controls (DCs, n = 251), untreated MS patients (uMS; n = 296), relapse-free treated MS patients (tMS; n = 78), and ProTEct-MS clinical trial participants (pMS; n = 41).
RESULTS
Using linear regression, we found a positive association between cNfL and age, as well as lower concentrations among women, in all groups, except for uMS patients. In contrast, disability level in the entire MS cohort, or T1 and T2 lesion volumes, brain parenchymal fraction, thalamic fraction, and cortical thickness in the pMS trial cohort, did not correlate with cNfL concentrations. Furthermore, the cNfL levels in tMS and pMS groups did not differ.
CONCLUSIONS
In participants with MS lacking signs of inflammatory disease activity, disease modulatory therapy reinstates an age and sex cNfL pattern similar to that of control subjects. No significant association was found between cNfL levels and clinical worsening, disability level, or MRI metrics.

Identifiants

pubmed: 36573731
doi: 10.1002/brb3.2873
pmc: PMC9847611
doi:

Substances chimiques

Biomarkers 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2873

Subventions

Organisme : University of Ostrava
ID : CZ.02.2.69/0.0/0.0/16_018/0002635
Organisme : GeNeuro S.A
ID : GeNeuro GNC-401/EudraCT number:
Organisme : GeNeuro S.A
ID : 2019-004822-15
Organisme : Swedish MRC
ID : 2020-02700
Organisme :  Hjärnfonden
ID : FO2021-0277

Informations de copyright

© 2022 The Authors. Brain and Behavior published by Wiley Periodicals LLC.

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Auteurs

Kamila Zondra Revendova (K)

Centre for Molecular Medicine, Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Centre for Neurology, Academic Specialist Center, Karolinska University Hospital, Stockholm, Sweden.
Department of Clinical Neurosciences, Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic.

Chiara Starvaggi Cucuzza (C)

Centre for Molecular Medicine, Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Centre for Neurology, Academic Specialist Center, Karolinska University Hospital, Stockholm, Sweden.

Ali Manouchehrinia (A)

Centre for Molecular Medicine, Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.

Mohsen Khademi (M)

Centre for Molecular Medicine, Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.

Michal Bar (M)

Department of Clinical Neurosciences, Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic.

David Leppert (D)

Neurologic Clinic and Policlinic, MS Center and Research Center for Clinical Neuroimmunology and Neuroscience Basel (RC2NB), Basel, Switzerland.

Elisabeth Sandberg (E)

Department of Neuroradiology, Karolinska University Hospital, Stockholm, Sweden.

Russell Ouellette (R)

Department of Neuroradiology, Karolinska University Hospital, Stockholm, Sweden.

Tobias Granberg (T)

Department of Neuroradiology, Karolinska University Hospital, Stockholm, Sweden.

Fredrik Piehl (F)

Centre for Molecular Medicine, Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Centre for Neurology, Academic Specialist Center, Karolinska University Hospital, Stockholm, Sweden.

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