Nef enhances HIV-1 replication and infectivity independently of SERINC5 in CEM T cells.
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
received:
23
09
2022
revised:
14
12
2022
accepted:
15
12
2022
pubmed:
29
12
2022
medline:
7
1
2023
entrez:
28
12
2022
Statut:
ppublish
Résumé
A primary function of HIV-1 Nef is the enhancement of viral infectivity and replication. Whether counteraction of the antiretroviral proteins SERINC3 and SERINC5 is the cause of this positive influence on viral growth-rate and infectivity remains unclear. Here, we utilized CRISPR/Cas9 to knockout SERINC3 and SERINC5 in a leukemic CD4-positive T cell line (CEM) that displays nef-related infectivity and growth-rate phenotypes. Viral replication was attenuated in CEM cells infected with HIV-1 lacking Nef (HIV-1ΔNef). This attenuated growth-rate phenotype was observed regardless of whether the coding regions of the serinc3 or serinc5 genes were intact. Moreover, knockout of serinc5 alone or of both serinc5 and serinc3 together failed to restore the infectivity of HIV1ΔNef virions produced from infected CEM cells. Our results corroborate a similar study using another T-lymphoid cell line (MOLT-3) and indicate that the antagonism of SERINC3 and SERINC5 does not fully explain the virology of HIV-1 lacking Nef.
Identifiants
pubmed: 36577173
pii: S0042-6822(22)00219-7
doi: 10.1016/j.virol.2022.12.008
pmc: PMC10484624
mid: NIHMS1919722
pii:
doi:
Substances chimiques
Membrane Proteins
0
nef Gene Products, Human Immunodeficiency Virus
0
nef protein, Human immunodeficiency virus 1
0
SERINC5 protein, human
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
154-162Subventions
Organisme : NIGMS NIH HHS
ID : K12 GM068524
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI129706
Pays : United States
Informations de copyright
Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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