Mass Spectrometry of Neutral Glycosphingolipids.
Ceramide structures
Glycan sequences
Glycosphingolipids (GSLs)
Liquid chromatography-electrospray ionization mass spectrometry (LC-ESI/MS)
Mass spectrometry (MS)
Multiple reaction monitoring (MRM)
Reversed phase liquid chromatography
Thin layer chromatography-matrix assisted laser desorption ionization/mass spectrometry (TLC-MALDI/MS)
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
entrez:
31
12
2022
pubmed:
1
1
2023
medline:
4
1
2023
Statut:
ppublish
Résumé
This chapter describes the protocols for mass spectrometry (MS) applied to the structural characterization of neutral glycosphingolipids (GSLs) and the determination of neutral GSL contents in biological materials. The structural characterization is performed by thin layer chromatography-matrix assisted laser desorption ionization/mass spectrometry (TLC-MALDI/MS) and liquid chromatography-electrospray ionization/mass spectrometry (LC-ESI/MS) with reversed phase separation. The content determination is carried out by LC-ESI/MS with multiple reaction monitoring (MRM). These protocols provide clues for the functions of neutral GSLs at the level of a single GSL molecular species.
Identifiants
pubmed: 36587076
doi: 10.1007/978-1-0716-2910-9_11
doi:
Substances chimiques
Neutral Glycosphingolipids
0
Glycosphingolipids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
127-144Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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