Quantification of particle number concentration in liposomal suspensions by Laser Transmission Spectroscopy (LTS).

Liposomes Suspensions Spectrum Analysis Dynamic Light Scattering Lasers Particle Size
Counting technique Extinction coefficient Laser Transmission Spectroscopy Liposomes Particle number concentration

Journal

Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Titre abrégé: Colloids Surf B Biointerfaces
Pays: Netherlands
ID NLM: 9315133

Informations de publication

Date de publication:
Feb 2023
Historique:
received: 04 11 2022
revised: 20 12 2022
accepted: 06 01 2023
pubmed: 15 1 2023
medline: 8 2 2023
entrez: 14 1 2023
Statut: ppublish

Résumé

Laser Transmission Spectroscopy (LTS) is an experimental technique able to determine the particle number concentration and the size of colloidal suspensions by a single measurement of the transmittance of a laser beam through the suspension of particles as a function of the wavelength. In this protocol, we show that LTS represents a unique and powerful tool to investigate suspensions of liposomes, where the precise quantification of the number concentration is particularly relevant for the complete definition of the colloidal properties of the suspension. We study a model formulation of Soy-PC:Chol liposomes and we validate LTS results by comparison with High-Performance Liquid Chromatography determination of lipid mass. Then LTS protocols is applied to state-of-art liposomal nanocarrier suspensions. We explain details of data analysis to obtain the particle number concentration by using the Lambert-Beer law and by calculating the extinction cross section, within the framework of Mie theory for spherical vesicles. We also determine the liposome radius and compare it with the hydrodynamic radius measured by Dynamic Light Scattering. As future perspective, we aim to extend LTS analysis to other nanostructures with different geometries and to contribute to the development of new quantitative strategies for the accurate characterization of nanocarriers and other nanoparticles.

Identifiants

DOI: 10.1016/j.colsurfb.2023.113137 PMID: 36640540
pubmed: 36640540
pii: S0927-7765(23)00015-2
doi: 10.1016/j.colsurfb.2023.113137
pii:
doi:

Substances chimiques

Liposomes 0
Suspensions 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

113137

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Simona Sennato (S)

Institute for Complex Systems, National Research Council (CNR-ISC), Sapienza University of Rome, Piazzale Aldo Moro 2, 00185, Rome, Italy; Physics Department, Sapienza University of Rome, Piazzale Aldo Moro 2, 00185, Rome, Italy. Electronic address: simona.sennato@roma1.infn.it.

Angelo Sarra (A)

Institute for Complex Systems, National Research Council (CNR-ISC), Sapienza University of Rome, Piazzale Aldo Moro 2, 00185, Rome, Italy; Microscopy Center, University of L'Aquila, Via Vetoio, Coppito, L' Aquila 67100, Italy.

Carlo Panella La Capria (CP)

Physics Department, Sapienza University of Rome, Piazzale Aldo Moro 2, 00185, Rome, Italy.

Cecilia Bombelli (C)

Institute for Biological Systems, National Research Council (ISB-CNR) Secondary Office of Rome-Reaction Mechanisms c/o Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy.

Enrica Donati (E)

Institute for Biological Systems, National Research Council (ISB-CNR), Rome1 Research Area, Strada Provinciale 35d 9, Montelibretti, Rome 00010, Italy.

Paolo Postorino (P)

Physics Department, Sapienza University of Rome, Piazzale Aldo Moro 2, 00185, Rome, Italy.

Federico Bordi (F)

Institute for Complex Systems, National Research Council (CNR-ISC), Sapienza University of Rome, Piazzale Aldo Moro 2, 00185, Rome, Italy; Physics Department, Sapienza University of Rome, Piazzale Aldo Moro 2, 00185, Rome, Italy.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › Liposomes › Quantification of particle number...
questionsmedicales.fr › Préparations pharmaceutiques › Colloïdes › Suspensions › Quantification of particle number...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Quantification of particle number...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Diffusion dynamique de la lumière › Quantification of particle number...
questionsmedicales.fr › Équipement et fournitures › Équipement et fournitures pour les applications des radiations › Lasers › Quantification of particle number...
questionsmedicales.fr › Phénomènes chimiques › Taille de particule › Quantification of particle number...