Electrocatalytic Aldehyde Oxidation by a Tungsten Dependent Aldehyde Oxidoreductase from Aromatoleum Aromaticum.
aldehyde
electrochemistry
enzyme
tungsten
Journal
Chemistry (Weinheim an der Bergstrasse, Germany)
ISSN: 1521-3765
Titre abrégé: Chemistry
Pays: Germany
ID NLM: 9513783
Informations de publication
Date de publication:
06 Apr 2023
06 Apr 2023
Historique:
received:
01
10
2022
medline:
7
4
2023
pubmed:
18
1
2023
entrez:
17
1
2023
Statut:
ppublish
Résumé
In contrast to their molybdenum dependent relatives, tungsten enzymes operate at significantly lower redox potentials, and in some cases they can carry out reversible redox transformations of their substrates and products. Still, the electrochemical properties of W enzymes have received much less attention than their Mo relatives. Herein we analyse the tungsten enzyme aldehyde oxidoreductase (AOR) from the mesophilic bacterium Aromatoleum aromaticum which has been immobilised on a glassy carbon working electrode. This generates a functional system that electrochemically oxidises a wide variety of aromatic and aliphatic aldehydes in the presence of the electron transfer mediators benzyl viologen, methylene blue or dichlorophenol indophenol. Simulation of the cyclic voltammetry has enabled a thorough kinetic analysis of the system, which reveals that methylene blue acts as a two-electron acceptor. In contrast, the other two mediators act as single electron oxidants. The different electrochemical driving forces imparted by these mediators also lead to significantly different outer sphere electron transfer rates with AOR. This work shows that electrocatalytic aldehyde oxidation can be achieved at a low applied electrochemical potential leading to an extremely energy efficient catalytic process.
Identifiants
pubmed: 36648073
doi: 10.1002/chem.202203072
doi:
Substances chimiques
Aldehyde Oxidoreductases
EC 1.2.-
Aldehydes
0
Tungsten
V9306CXO6G
Methylene Blue
T42P99266K
Aldehyde Dehydrogenase
EC 1.2.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202203072Subventions
Organisme : Australian Research Council
ID : DP220103268
Organisme : Deutsche Forschungsgemeinschaft
ID : He2190/11-1
Organisme : EU Project POWR
ID : 03.02.00-00-I004/16
Informations de copyright
© 2023 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH.
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