Mining and Characterization of Thermophilic Glucose Isomerase Based on Virtual Probe Technology.


Journal

Applied biochemistry and biotechnology
ISSN: 1559-0291
Titre abrégé: Appl Biochem Biotechnol
Pays: United States
ID NLM: 8208561

Informations de publication

Date de publication:
Jul 2023
Historique:
accepted: 10 01 2023
medline: 3 7 2023
pubmed: 26 1 2023
entrez: 25 1 2023
Statut: ppublish

Résumé

Fructose, which is produced by the isomerization of glucose isomerase, is a crucial precursor for the biosynthesis of rare sugars. In this study, thermophilic glucose isomerases (GI) from Caldicellulosiruptor acetigenus (CAGI), Thermoanaerobacter thermocopriae (TTGI), and Thermotoga petrophila (TPGI) were screened from GenBank database by a virtual probe and were successfully expressed in Escherichia coli BL21(DE3). The results of characterization demonstrated that the optimal pH for CAGI and TTGI were 8.0 and were maintained at 80% in a slightly acidic environment. The relative residual activities of CAGI and TTGI were found to be 40.6% and 52.6%, respectively, following an incubation period of 24 h at 90 ℃. Furthermore, CAGI and TTGI exhibited superior catalytic performance that their reaction equilibrium both reached only after an hour at 85 ℃ with 200 g/L glucose, and the highest conversion rates were 54.2% and 54.1%, respectively. This study identifies competitive enzyme candidates for fructose production in the industry with appreciable cost reduction.

Identifiants

pubmed: 36696038
doi: 10.1007/s12010-023-04349-5
pii: 10.1007/s12010-023-04349-5
doi:

Substances chimiques

xylose isomerase EC 5.3.1.5
Glucose IY9XDZ35W2
Fructose 30237-26-4
Aldose-Ketose Isomerases EC 5.3.1.-
Recombinant Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

4399-4413

Subventions

Organisme : the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang
ID : 2018R01014
Organisme : Innovation Demonstration Special Project of Henan
ID : 201111110100
Organisme : Major Science and Technology Projects for Public Welfare of Henan
ID : 201300110300
Organisme : Key Project Foundation of Natural Science Research of Universities of Henan
ID : 20A550004
Organisme : Fundamental Research Funds for the Henan
ID : 2018QNJH13
Organisme : High-Level Talents Research Fund of HAUT
ID : 2020BS064

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Yu-Qi Dong (YQ)

School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, People's Republic of China.
The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.

Ji-Dong Shen (JD)

The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.

Long Pan (L)

School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, People's Republic of China.
Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat, Zhengzhou, 450001, People's Republic of China.

Ji-Hong Huang (JH)

School of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, People's Republic of China. huangjih1216@126.com.
Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat, Zhengzhou, 450001, People's Republic of China. huangjih1216@126.com.

Zhi-Qiang Liu (ZQ)

The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China. microliu@zjut.edu.cn.

Yu-Guo Zheng (YG)

The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.

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