Oligoribonucleotide-Mediated Blockade of DNA Extension by
Journal
Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536
Informations de publication
Date de publication:
14 02 2023
14 02 2023
Historique:
pubmed:
5
2
2023
medline:
16
2
2023
entrez:
4
2
2023
Statut:
ppublish
Résumé
Blocking PCR is a method that inhibits amplification of DNA possessing a nucleotide sequence complementary to that of a blocker; the method can be used to suppress amplification of target wild-type DNA while amplifying mutated DNA. Previously, we demonstrated that an oligoribonucleotide (ORN) functions as a cost-effective and sequence-specific blocker. This blocking PCR system, named ORN interference-PCR (ORNi-PCR), is compatible with DNA polymerases lacking 5'-3' exonuclease activity but not with those possessing the activity (e.g.,
Identifiants
pubmed: 36738294
doi: 10.1021/acs.analchem.2c05186
doi:
Substances chimiques
Taq Polymerase
EC 2.7.7.-
Oligoribonucleotides
0
Nucleotides
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM