Role of membrane mimetics on biophysical EPR studies of membrane proteins.
Detergent micelles
EPR spectroscopy
Lipid bilayers
Lipodisq nanoparticles
Membrane mimetics
SMA copolymer
Structural dynamics
Journal
Biochimica et biophysica acta. Biomembranes
ISSN: 1879-2642
Titre abrégé: Biochim Biophys Acta Biomembr
Pays: Netherlands
ID NLM: 101731713
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
received:
06
12
2022
revised:
01
02
2023
accepted:
03
02
2023
pubmed:
11
2
2023
medline:
15
3
2023
entrez:
10
2
2023
Statut:
ppublish
Résumé
Biological membranes are essential in providing the stability of membrane proteins in a functional state. Functionally stable homogeneous sample is required for biophysical electron paramagnetic resonance (EPR) studies of membrane proteins for obtaining pertinent structural dynamics of the protein. Significant progresses have been made for the optimization of the suitable membrane environments required for biophysical EPR measurements. However, no universal membrane mimetic system is available that can solubilize all membrane proteins suitable for biophysical EPR studies while maintaining the functional integrity. Great efforts are needed to optimize the sample condition to obtain better EPR data quality of membrane proteins that can provide meaningful information on structural dynamics. In this mini-review, we will discuss important aspects of membrane mimetics for biophysical EPR measurements and current progress with some of the recent examples.
Identifiants
pubmed: 36764474
pii: S0005-2736(23)00020-2
doi: 10.1016/j.bbamem.2023.184138
pii:
doi:
Substances chimiques
Membrane Proteins
0
Types de publication
Journal Article
Review
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
184138Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM126935
Pays : United States
Informations de copyright
Copyright © 2023 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.