Metal-responsive elements confer cadmium response in Arabidopsis.
Arabidopsis
Cadmium
Metal-responsive element
Transcription factor
Journal
Planta
ISSN: 1432-2048
Titre abrégé: Planta
Pays: Germany
ID NLM: 1250576
Informations de publication
Date de publication:
11 Feb 2023
11 Feb 2023
Historique:
received:
03
11
2022
accepted:
07
02
2023
entrez:
11
2
2023
pubmed:
12
2
2023
medline:
15
2
2023
Statut:
epublish
Résumé
Molecular, biochemical, and genetic experiments demonstrate that metal-responsive elements (MREs), initially identified in animals, confer the cadmium transcriptional response in Arabidopsis, thus providing deep functional insights of MREs in plants. Cadmium (Cd) is highly toxic to all organisms including plants. Cd-responsive gene transcription is a fundamental aspect of the Cd response, in which Cd stress regulatory cis-acting elements are essential. However, little is known regarding such elements in plants. Metal-responsive elements (MREs, 5'-TGCRCNC-3', R: A or G, N: any base) are essential for transcriptional induction of Cd in animals. MREs are also contained in the promoters of some Cd-regulated plant genes, but whether MREs confer Cd responses in plants is poorly defined. Herein, we used a previously identified MRE of the tobacco feedback-insensitive anthranilate synthase α-2 chain gene as a representative MRE (named as MREa, 5'-TGCACAC-3') to explore the roles of MREs in the transcriptional response to Cd stress in Arabidopsis thaliana. First, we showed that MREa conferred Cd stress responsiveness on a minimal promoter in both concentration- and time-dependent manners, whereas the mutated MREa did not. Second, MREa specifically bound nuclear extracts, displaying a biochemical characteristic of cis-acting elements. We screened and identified four MREa-binding transcription factors, including ethylene response factor 13 (AtERF13). At last, MREa could mediate AtERF13 to activate the β-glucuronidase (GUS) reporter expression. Overall, these molecular, biochemical, and genetic data suggest that MREa is instrumental in the Cd response in Arabidopsis, thus providing deep functional insights of MREs in plants.
Identifiants
pubmed: 36773095
doi: 10.1007/s00425-023-04093-4
pii: 10.1007/s00425-023-04093-4
doi:
Substances chimiques
Cadmium
00BH33GNGH
Metals
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
53Subventions
Organisme : National Natural Science Foundation of China
ID : 31771361
Organisme : National Natural Science Foundation of China
ID : 32070546
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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