Emergence of mass spectrometry detergents for membrane proteomics.
Detergent
Mass spectrometry
Membrane
Omics
Protein
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Jul 2023
Jul 2023
Historique:
received:
12
11
2022
accepted:
02
02
2023
revised:
25
01
2023
medline:
10
7
2023
pubmed:
23
2
2023
entrez:
22
2
2023
Statut:
ppublish
Résumé
Detergents enable the investigation of membrane proteins by mass spectrometry. Detergent designers aim to improve underlying methodologies and are confronted with the challenge to design detergents with optimal solution and gas-phase properties. Herein, we review literature related to the optimization of detergent chemistry and handling and identify an emerging research direction: the optimization of mass spectrometry detergents for individual applications in mass spectrometry-based membrane proteomics. We provide an overview about qualitative design aspects including their relevance for the optimization of detergents in bottom-up proteomics, top-down proteomics, native mass spectrometry, and Nativeomics. In addition to established design aspects, such as charge, concentration, degradability, detergent removal, and detergent exchange, it becomes apparent that detergent heterogeneity is a promising key driver for innovation. We anticipate that rationalizing the role of detergent structures in membrane proteomics will serve as an enabling step for the analysis of challenging biological systems.
Identifiants
pubmed: 36808272
doi: 10.1007/s00216-023-04584-z
pii: 10.1007/s00216-023-04584-z
pmc: PMC10328889
doi:
Substances chimiques
Detergents
0
Membrane Proteins
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
3897-3909Subventions
Organisme : Ministry of Culture and Science of the State of North Rhine-Westphalia
ID : NRW return program
Informations de copyright
© 2023. The Author(s).
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