Identification of RimR2 as a positive pathway-specific regulator of rimocidin biosynthesis in Streptomyces rimosus M527.

Bacterial Proteins / metabolism Gene Expression Regulation, Bacterial Phylogeny Polyenes / metabolism Streptomyces rimosus / metabolism
LAL regulator RimR2 Rimocidin Streptomyces rimosus

Journal

Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812

Informations de publication

Date de publication:
21 Feb 2023
Historique:
received: 23 10 2022
accepted: 10 02 2023
entrez: 22 2 2023
pubmed: 23 2 2023
medline: 25 2 2023
Statut: epublish

Résumé

Streoptomyces rimosus M527 is a producer of the polyene macrolide rimocidin which shows activity against various plant pathogenic fungi. Notably, the regulatory mechanisms underlying rimocidin biosynthesis are yet to be elucidated. In this study, using domain structure and amino acid alignment and phylogenetic tree construction, rimR2, which located in the rimocidin biosynthetic gene cluster, was first found and identified as a larger ATP-binding regulators of the LuxR family (LAL) subfamily regulator. The rimR2 deletion and complementation assays were conducted to explore its role. Mutant M527-ΔrimR2 lost its ability to produce rimocidin. Complementation of M527-ΔrimR2 restored rimocidin production. The five recombinant strains, M527-ER, M527-KR, M527-21R, M527-57R, and M527-NR, were constructed by overexpressing rimR2 gene using the promoters permE A LAL regulator RimR2 was identified as a positive specific-pathway regulator of rimocidin biosynthesis in M527. RimR2 regulates the rimocidin biosynthesis by influencing the transcriptional levels of rim genes and binding to the promoter regions of rimA and rimC.

Sections du résumé

BACKGROUND BACKGROUND
Streoptomyces rimosus M527 is a producer of the polyene macrolide rimocidin which shows activity against various plant pathogenic fungi. Notably, the regulatory mechanisms underlying rimocidin biosynthesis are yet to be elucidated.
RESULTS RESULTS
In this study, using domain structure and amino acid alignment and phylogenetic tree construction, rimR2, which located in the rimocidin biosynthetic gene cluster, was first found and identified as a larger ATP-binding regulators of the LuxR family (LAL) subfamily regulator. The rimR2 deletion and complementation assays were conducted to explore its role. Mutant M527-ΔrimR2 lost its ability to produce rimocidin. Complementation of M527-ΔrimR2 restored rimocidin production. The five recombinant strains, M527-ER, M527-KR, M527-21R, M527-57R, and M527-NR, were constructed by overexpressing rimR2 gene using the promoters permE
CONCLUSION CONCLUSIONS
A LAL regulator RimR2 was identified as a positive specific-pathway regulator of rimocidin biosynthesis in M527. RimR2 regulates the rimocidin biosynthesis by influencing the transcriptional levels of rim genes and binding to the promoter regions of rimA and rimC.

Identifiants

DOI: 10.1186/s12934-023-02039-9 PMID: 36810073 PMC: PMC9942304
pubmed: 36810073
doi: 10.1186/s12934-023-02039-9
pii: 10.1186/s12934-023-02039-9
pmc: PMC9942304
doi:

Substances chimiques

Bacterial Proteins 0
Polyenes 0
rimocidin 72LLC1Q06O

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

32

Informations de copyright

© 2023. The Author(s).

Références

PLoS One. 2012;7(10):e46758
pubmed: 23056438
Nat Biotechnol. 2003 May;21(5):526-31
pubmed: 12692562
Eur J Biochem. 2004 Mar;271(6):1198-208
pubmed: 15009198
Biochem Pharmacol. 2017 Jun 1;133:86-96
pubmed: 27884742
Appl Microbiol Biotechnol. 2020 May;104(10):4445-4455
pubmed: 32221690
Curr Opin Biotechnol. 2014 Oct;29:107-15
pubmed: 24762576
Appl Microbiol Biotechnol. 2020 Dec;104(23):10191-10202
pubmed: 33057790
Appl Microbiol Biotechnol. 2005 Jun;67(4):436-43
pubmed: 15700127
Metab Eng. 2011 Nov;13(6):756-67
pubmed: 22001323
J Biol Chem. 2011 Mar 18;286(11):9150-61
pubmed: 21187288
Microbiol Res. 2014 Jul-Aug;169(7-8):602-8
pubmed: 24231162
J Am Chem Soc. 2010 Dec 29;132(51):18266-72
pubmed: 21126070
PLoS One. 2012;7(6):e38536
pubmed: 22693644
Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20404-9
pubmed: 19073939
Appl Microbiol Biotechnol. 2019 Sep;103(18):7583-7596
pubmed: 31327020
Mol Microbiol. 2005 Mar;55(6):1855-66
pubmed: 15752205
PLoS One. 2015 Aug 18;10(8):e0135891
pubmed: 26284936
mSystems. 2021 May 4;6(3):
pubmed: 33947798
Biotechnol Adv. 2019 Nov 1;37(6):107366
pubmed: 30853630
Microbiology (Reading). 2007 Sep;153(Pt 9):3174-3183
pubmed: 17768260
Mol Microbiol. 1997 Sep;25(6):1181-4
pubmed: 9350875
Metab Eng. 2018 Nov;50:74-84
pubmed: 29852270
J Nat Prod. 2020 Jun 26;83(6):1919-1924
pubmed: 32519857
J Biol Chem. 2015 Mar 20;290(12):7992-8001
pubmed: 25648897
J Basic Microbiol. 2016 Aug;56(8):929-33
pubmed: 27192632
J Ind Microbiol Biotechnol. 2019 May;46(5):697-708
pubmed: 30697650
J Bacteriol. 2004 May;186(9):2567-75
pubmed: 15090496
Curr Opin Microbiol. 2010 Jun;13(3):263-73
pubmed: 20303823
Chem Biol. 2001 Jul;8(7):713-23
pubmed: 11451671
Appl Microbiol Biotechnol. 2016 May;100(9):3893-908
pubmed: 27023916
Gene. 2004 Dec 8;343(1):107-15
pubmed: 15563836
J Ind Microbiol Biotechnol. 2023 Feb 13;49(6):
pubmed: 36572395
Metab Eng. 2013 Sep;19:98-106
pubmed: 23876413
Appl Environ Microbiol. 2020 Apr 17;86(9):
pubmed: 32086301
Appl Microbiol Biotechnol. 2019 Sep;103(17):7071-7084
pubmed: 31256228
Nat Biotechnol. 2020 Jan;38(1):76-83
pubmed: 31819261
Curr Opin Biotechnol. 2021 Jun;69:26-34
pubmed: 33316577
Microbiology (Reading). 2015 Mar;161(Pt 3):539-52
pubmed: 25575546
J Appl Microbiol. 2016 May;120(5):1219-28
pubmed: 26808253
Chem Biol. 2000 Jun;7(6):395-403
pubmed: 10873841
Microb Cell Fact. 2018 Jun 19;17(1):98
pubmed: 29914489
J Zhejiang Univ Sci B. 2019 Nov.;20(11):891-900
pubmed: 31595725
Appl Microbiol Biotechnol. 2014 Nov;98(22):9311-24
pubmed: 25104037
Microbiol Res. 2022 Sep;262:127077
pubmed: 35688098
Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9509-14
pubmed: 10449723
Appl Microbiol Biotechnol. 2015 Dec;99(24):10575-85
pubmed: 26318449
Appl Environ Microbiol. 2013 Jul;79(14):4484-92
pubmed: 23686264
Microb Cell Fact. 2016 Sep 07;15(1):152
pubmed: 27604457
Appl Environ Microbiol. 2021 Apr 27;87(10):
pubmed: 33637575
Antibiotics (Basel). 2022 Jul 24;11(8):
pubmed: 35892384
J Bacteriol. 2004 Mar;186(5):1345-54
pubmed: 14973031
Chem Biol. 2004 Mar;11(3):357-66
pubmed: 15123265
J Ind Microbiol Biotechnol. 2020 Oct;47(9-10):739-752
pubmed: 32778981
Appl Microbiol Biotechnol. 2015 Jun;99(12):5123-35
pubmed: 25715784
Microbiol Mol Biol Rev. 2013 Mar;77(1):112-43
pubmed: 23471619

Auteurs

Huijie Li (H)

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Xueyuan Street, Xiasha Higher Education District, Hangzhou, 310018, Zhejiang, People's Republic of China.

Yefeng Hu (Y)

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Xueyuan Street, Xiasha Higher Education District, Hangzhou, 310018, Zhejiang, People's Republic of China.

Yongyong Zhang (Y)

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Xueyuan Street, Xiasha Higher Education District, Hangzhou, 310018, Zhejiang, People's Republic of China.

Zheng Ma (Z)

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Xueyuan Street, Xiasha Higher Education District, Hangzhou, 310018, Zhejiang, People's Republic of China. mazheng520@163.com.

Andreas Bechthold (A)

Institute for Pharmaceutical Sciences, Pharmaceutical Biology and Biotechnology, University of Freiburg, 79104, Freiburg, Germany.

Xiaoping Yu (X)

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Xueyuan Street, Xiasha Higher Education District, Hangzhou, 310018, Zhejiang, People's Republic of China. yuxiaoping19630306@163.com.

Articles similaires

Comprehensive comparative analysis and development of molecular markers for Lasianthus species based on complete chloroplast genome sequences.

Yue Zhang, Meifang Song, Deying Tang et al.
1.00
Genome, Chloroplast Phylogeny Genetic Markers Base Composition High-Throughput Nucleotide Sequencing

Atomic view of photosynthetic metabolite permeability pathways and confinement in synthetic carboxysome shells.

Daipayan Sarkar, Christopher Maffeo, Markus Sutter et al.
1.00
Photosynthesis Ribulose-Bisphosphate Carboxylase Carbon Dioxide Molecular Dynamics Simulation Cyanobacteria

Decoding the genomic terrain: functional insights into 14 chemosensory proteins in whitefly Bemisia tabaci Asia II-1.

M N Rudra Gouda, Sabtharishi Subramanian
1.00
Animals Hemiptera Insect Proteins Phylogeny Insecticides

Initial pH determines the morphological characteristics and secondary metabolite production in Aspergillus terreus and Streptomyces rimosus cocultures.

Tomasz Boruta, Martyna Foryś, Weronika Pawlikowska et al.
1.00
Aspergillus Hydrogen-Ion Concentration Coculture Techniques Secondary Metabolism Streptomyces rimosus

Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Identification of RimR2 as a positive...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › Identification of RimR2 as a positive...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Identification of RimR2 as a positive...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures acycliques › Alcènes › Polyènes › Identification of RimR2 as a positive...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Streptomycetaceae › Streptomyces › Streptomyces rimosus › Identification of RimR2 as a positive...