Complete sequencing of a cynomolgus macaque major histocompatibility complex haplotype.
Journal
Genome research
ISSN: 1549-5469
Titre abrégé: Genome Res
Pays: United States
ID NLM: 9518021
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
received:
24
10
2022
accepted:
21
02
2023
medline:
15
6
2023
pubmed:
1
3
2023
entrez:
28
2
2023
Statut:
ppublish
Résumé
Macaques provide the most widely used nonhuman primate models for studying the immunology and pathogenesis of human diseases. Although the macaque major histocompatibility complex (MHC) region shares most features with the human leukocyte antigen (HLA) region, macaques have an expanded repertoire of MHC class I genes. Although a chimera of two rhesus macaque MHC haplotypes was first published in 2004, the structural diversity of MHC genomic organization in macaques remains poorly understood owing to a lack of adequate genomic reference sequences. We used ultralong Oxford Nanopore and high-accuracy Pacific Biosciences (PacBio) HiFi sequences to fully assemble the ∼5.2-Mb M3 haplotype of an MHC-homozygous, Mauritian-origin cynomolgus macaque (
Identifiants
pubmed: 36854669
pii: gr.277429.122
doi: 10.1101/gr.277429.122
pmc: PMC10078292
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
448-462Subventions
Organisme : NLM NIH HHS
ID : T15 LM007359
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM140935
Pays : United States
Informations de copyright
© 2023 Karl et al.; Published by Cold Spring Harbor Laboratory Press.