Backbone NMR assignment of the yeast expressed Fab fragment of the NISTmAb reference antibody.

Immunoglobulin Fab Fragments / chemistry Saccharomyces cerevisiae Nuclear Magnetic Resonance, Biomolecular Antibodies, Monoclonal / chemistry Magnetic Resonance Spectroscopy
Backbone assignments Biologics Fab domain NISTmAb

Journal

Biomolecular NMR assignments
ISSN: 1874-270X
Titre abrégé: Biomol NMR Assign
Pays: Netherlands
ID NLM: 101472371

Informations de publication

Date de publication:
06 2023
Historique:
received: 21 11 2022
accepted: 08 02 2023
medline: 2 6 2023
pubmed: 2 3 2023
entrez: 1 3 2023
Statut: ppublish

Résumé

The monoclonal antibody (mAb) protein class has become a primary therapeutic platform for the production of new life saving drug products. MAbs are comprised of two domains: the antigen-binding fragment (Fab) and crystallizable fragment (Fc). Despite the success in the clinic, NMR assignments of the complete Fab domain have been elusive, in part due to problems in production of properly folded, triply-labeled

Identifiants

DOI: 10.1007/s12104-023-10123-9 PMID: 36856943
pubmed: 36856943
doi: 10.1007/s12104-023-10123-9
pii: 10.1007/s12104-023-10123-9
doi:

Substances chimiques

Immunoglobulin Fab Fragments 0
Antibodies, Monoclonal 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

75-81

Informations de copyright

© 2023. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.

Références

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Auteurs

Tsega L Solomon (TL)

Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, MD, 20850, USA.
ORCID: 0000-0003-1242-2297

Kinlin Chao (K)

Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, MD, 20850, USA.
ORCID: 0000-0003-3931-4608

Genevieve Gingras (G)

Centre for Oncology, Radiopharmaceuticals and Research, Biologics and Radiotherapeutic Drugs Directorate, Health Canada, 251 Sir Frederick Banting Driveway, K1A 0K9, Ottawa, ON, Canada.

Yves Aubin (Y)

Centre for Oncology, Radiopharmaceuticals and Research, Biologics and Radiotherapeutic Drugs Directorate, Health Canada, 251 Sir Frederick Banting Driveway, K1A 0K9, Ottawa, ON, Canada.
ORCID: 0000-0002-8554-0297

William B O'Dell (WB)

Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, MD, 20850, USA.
ORCID: 0000-0002-8063-5190

John P Marino (JP)

Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, MD, 20850, USA.
ORCID: 0000-0002-6860-5853

Robert G Brinson (RG)

Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, MD, 20850, USA. robert.brinson@nist.gov.
ORCID: 0000-0001-5424-990X

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Fragments d'immunoglobuline › Fragments Fab d'immunoglobuline › Backbone NMR assignment of the yeast expressed...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › Backbone NMR assignment of the yeast expressed...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectroscopie par résonance magnétique › Résonance magnétique nucléaire biomoléculaire › Backbone NMR assignment of the yeast expressed...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps monoclonaux › Backbone NMR assignment of the yeast expressed...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectroscopie par résonance magnétique › Backbone NMR assignment of the yeast expressed...