Structure and mechanism of the plant RNA polymerase V.
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
24 03 2023
24 03 2023
Historique:
pubmed:
10
3
2023
medline:
28
3
2023
entrez:
9
3
2023
Statut:
ppublish
Résumé
In addition to the conserved RNA polymerases I to III (Pols I to III) in eukaryotes, two atypical polymerases, Pols IV and V, specifically produce noncoding RNA in the RNA-directed DNA methylation pathway in plants. Here, we report on the structures of cauliflower Pol V in the free and elongation conformations. A conserved tyrosine residue of NRPE2 stacks with a double-stranded DNA branch of the transcription bubble to potentially attenuate elongation by inducing transcription stalling. The nontemplate DNA strand is captured by NRPE2 to enhance backtracking, thereby increasing 3'-5' cleavage, which likely underpins Pol V's high fidelity. The structures also illuminate the mechanism of Pol V transcription stalling and enhanced backtracking, which may be important for Pol V's retention on chromatin to serve its function in tethering downstream factors for RNA-directed DNA methylation.
Identifiants
pubmed: 36893216
doi: 10.1126/science.adf8231
pmc: PMC10041816
mid: NIHMS1881983
doi:
Substances chimiques
DNA-Directed RNA Polymerases
EC 2.7.7.6
RNA, Plant
0
Plant Proteins
0
RNA, Untranslated
0
DNA, Plant
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1209-1213Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM130272
Pays : United States
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