Structure of the lipopolysaccharide O-antigen of endophytic Pseudomonas sp. strain L1.
Endophytes
Lipopolysaccharide
MALDI-TOF
NMR
O-specific polysaccharide
Pseudomonas sp
Journal
Carbohydrate research
ISSN: 1873-426X
Titre abrégé: Carbohydr Res
Pays: Netherlands
ID NLM: 0043535
Informations de publication
Date de publication:
Mar 2023
Mar 2023
Historique:
received:
30
01
2023
accepted:
22
02
2023
pubmed:
10
3
2023
medline:
21
3
2023
entrez:
9
3
2023
Statut:
ppublish
Résumé
The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Pseudomonas sp. Strain L1, the endophytic bacteria of Lolium perenne (ryegrass) plants growing in soil in an industrial area in the Silesia region (Zabrze, Southern Poland). The high-molecular-weight O-PS fraction liberated from Pseudomonas sp. L1 lipopolysaccharide by mild acid hydrolysis was studied using chemical methods, MALDI-TOF mass spectrometry, and 1D and 2D NMR spectroscopy techniques. It was found that the O-specific polysaccharide was built of tetrasaccharide repeating units composed of d-FucpN, d-Fucp4N, and two d-QuipN residues. The following structure of the O-PS of Pseudomonas sp. Strain L1 was established: [Formula: see text].
Identifiants
pubmed: 36893494
pii: S0008-6215(23)00041-1
doi: 10.1016/j.carres.2023.108779
pii:
doi:
Substances chimiques
O Antigens
0
Lipopolysaccharides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108779Informations de copyright
Copyright © 2023. Published by Elsevier Ltd.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.