Two-Step Validation Approach for Tools To Study the DNA Repair Enzyme SNM1A.
DNA repair
SNM1A
fragment-based screening
metal-binding groups
oligonucleotide modification
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
03 07 2023
03 07 2023
Historique:
revised:
06
03
2023
received:
16
12
2022
medline:
5
7
2023
pubmed:
15
3
2023
entrez:
14
3
2023
Statut:
ppublish
Résumé
We report a two-step validation approach to evaluate the suitability of metal-binding groups for targeting DNA damage-repair metalloenzymes using model enzyme SNM1A. A fragment-based screening approach was first used to identify metal-binding fragments suitable for targeting the enzyme. Effective fragments were then incorporated into oligonucleotides using the copper-catalysed azide-alkyne cycloaddition reaction. These modified oligonucleotides were recognised by SNM1A at >1000-fold lower concentrations than their fragment counterparts. The exonuclease SNM1A is a key enzyme involved in the repair of interstrand crosslinks, a highly cytotoxic form of DNA damage. However, SNM1A and other enzymes of this class are poorly understood, as there is a lack of tools available to facilitate their study. Our novel approach of incorporating functional fragments into oligonucleotides is broadly applicable to generating modified oligonucleotide structures with high affinity for DNA damage-repair enzymes.
Identifiants
pubmed: 36917742
doi: 10.1002/cbic.202200756
doi:
Substances chimiques
Exodeoxyribonucleases
EC 3.1.-
Cell Cycle Proteins
0
DNA Repair Enzymes
EC 6.5.1.-
Oligonucleotides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202200756Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
© 2023 The Authors. ChemBioChem published by Wiley-VCH GmbH.
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