A novel function for eukaryotic elongation factor 3: Inhibition of stop codon readthrough in yeast.

Saccharomyces cerevisiae / genetics Codon, Terminator / metabolism Peptide Elongation Factors / genetics Protein Biosynthesis Ribosomes / metabolism
Eukaryotic translation Stop codon readthrough eEF3

Journal

Archives of biochemistry and biophysics
ISSN: 1096-0384
Titre abrégé: Arch Biochem Biophys
Pays: United States
ID NLM: 0372430

Informations de publication

Date de publication:
15 05 2023
Historique:
received: 26 11 2022
revised: 16 03 2023
accepted: 18 03 2023
medline: 1 5 2023
pubmed: 23 3 2023
entrez: 22 3 2023
Statut: ppublish

Résumé

Eukaryotic elongation factor 3 (eEF3) is one of the essential yeast ribosome-associated ATP-binding cassette type F (ABCF) ATPases. Previously, we found that eEF3 stimulates release of mRNA from puromycin-treated polysomes. In this study, we used a cell-free cricket paralysis virus (CrPV) internal ribosome entry site (IRES)-mediated firefly luciferase bicistronic mRNA translation system with yeast S30 extract. When eEF3 was partially removed from the crude extract, the product from the downstream ORF was increased by the readthrough of a UAA stop codon in the upstream ORF. eEF3 enhanced the release of luciferase from the polysome by eukaryotic release factor (eRF)1 and eRF3. These results suggest that eEF3 is a factor that assists eRFs in performing normal protein synthesis termination in yeast.

Identifiants

DOI: 10.1016/j.abb.2023.109580 PMID: 36948349
pubmed: 36948349
pii: S0003-9861(23)00079-6
doi: 10.1016/j.abb.2023.109580
pii:
doi:

Substances chimiques

Codon, Terminator 0
Peptide Elongation Factors 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

109580

Informations de copyright

Copyright © 2023. Published by Elsevier Inc.

Déclaration de conflit d'intérêts

Declaration of competing interest There is no conflict of interest.

Auteurs

Soushi Kobayashi (S)

Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, 3610 Hamilton Walk, Philadelphia, PA, 19104, USA; Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, 19107, USA. Electronic address: soushik@pharm.showa-u.ac.jp.

Akira Kaji (A)

Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, 3610 Hamilton Walk, Philadelphia, PA, 19104, USA. Electronic address: kaji@pennmedicine.upenn.edu.

Hideko Kaji (H)

Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, 19107, USA.

Articles similaires

Saccharomyces cerevisiae whole cell biotransformation for the production of aldehyde flavors and fragrances.

Nazreen V M Abdul Muthaliff, Yao Zong Ng, Wei Mei Guo et al.
1.00
Saccharomyces cerevisiae Aldehydes Biotransformation Flavoring Agents Lipoxygenase

The dual life of disordered lysine-rich domains of snoRNPs in rRNA modification and nucleolar compaction.

Carine Dominique, Nana Kadidia Maiga, Alfonso Méndez-Godoy et al.
1.00
Saccharomyces cerevisiae Lysine Cell Nucleolus RNA, Ribosomal Saccharomyces cerevisiae Proteins

Minimisation of metabolic networks defines a new functional class of genes.

Giorgio Jansen, Tanda Qi, Vito Latora et al.
1.00
Metabolic Networks and Pathways Saccharomyces cerevisiae Computational Biology Synthetic Biology Computer Simulation

Effects of Pichia manshurica yeast supplementation on ruminal fermentation, nutrient degradability, and greenhouse gas emissions in aflatoxin B1 contaminated diets.

Yosra Soltan, Amr Morsy, Mahmoud Elazab et al.
1.00
Animals Rumen Fermentation Animal Feed Diet

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › A novel function for eukaryotic elongation...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Codon › Codon stop › A novel function for eukaryotic elongation...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines ribosomiques › Facteurs élongation chaîne peptidique › A novel function for eukaryotic elongation...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Biosynthèse des protéines › A novel function for eukaryotic elongation...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Ribosomes › A novel function for eukaryotic elongation...