FRETing about the details: Case studies in the use of a genetically encoded fluorescent amino acid for distance-dependent energy transfer.
LexA
alphasynuclein
energy transfer
fluorescence
non-canonical amino acid
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
05 2023
05 2023
Historique:
revised:
22
03
2023
received:
01
01
2023
accepted:
24
03
2023
pmc-release:
01
05
2024
medline:
1
5
2023
pubmed:
29
3
2023
entrez:
28
3
2023
Statut:
ppublish
Résumé
Förster resonance energy transfer (FRET) is a valuable method for monitoring protein conformation and biomolecular interactions. Intrinsically fluorescent amino acids that can be genetically encoded, such as acridonylalanine (Acd), are particularly useful for FRET studies. However, quantitative interpretation of FRET data to derive distance information requires careful use of controls and consideration of photophysical effects. Here we present two case studies illustrating how Acd can be used in FRET experiments to study small molecule induced conformational changes and multicomponent biomolecular complexes.
Identifiants
pubmed: 36974585
doi: 10.1002/pro.4633
pmc: PMC10108435
doi:
Substances chimiques
Amino Acids
0
Fluorescent Dyes
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4633Subventions
Organisme : NINDS NIH HHS
ID : U19 NS110456
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM127593
Pays : United States
Organisme : NIH HHS
ID : S10 OD030460
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS103873
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM133398
Pays : United States
Organisme : NIA NIH HHS
ID : T32 AG000255
Pays : United States
Informations de copyright
© 2023 The Protein Society.
Références
Trends Biochem Sci. 2016 Jun;41(6):491-507
pubmed: 27156117
Micromachines (Basel). 2021 Apr 27;12(5):
pubmed: 33925350
Anal Biochem. 1994 Apr;218(1):1-13
pubmed: 8053542
Nucleic Acids Res. 2013 Nov;41(21):9901-10
pubmed: 23965307
Cell. 2001 Sep 7;106(5):585-94
pubmed: 11551506
Bioorg Med Chem Lett. 2014 Jul 15;24(14):3194-8
pubmed: 24835632
Org Biomol Chem. 2016 Feb 7;14(5):1584-92
pubmed: 26695131
Methods Enzymol. 2022;664:151-171
pubmed: 35331372
Nature. 2015 Jun 18;522(7556):340-4
pubmed: 26061766
Pharmaceuticals (Basel). 2021 Aug 26;14(9):
pubmed: 34577548
Biochemistry. 2015 Jun 16;54(23):3573-82
pubmed: 26016604
Chem Sci. 2021 Aug 3;12(36):11955-11964
pubmed: 34976337
Biochemistry. 2022 Dec 20;61(24):2884-2896
pubmed: 36473084
Angew Chem Int Ed Engl. 2009;48(38):6974-98
pubmed: 19714693
Elife. 2018 Jul 03;7:
pubmed: 29969391
Curr Opin Struct Biol. 2008 Feb;18(1):16-26
pubmed: 18221865
Elife. 2021 Oct 08;10:
pubmed: 34623258
Methods Enzymol. 2020;639:37-69
pubmed: 32475410
Biochemistry. 2017 Feb 7;56(5):683-691
pubmed: 28045494
ACS Chem Neurosci. 2018 Nov 21;9(11):2521-2527
pubmed: 29750499
Biophys J. 2017 Nov 21;113(10):2182-2191
pubmed: 28939194
Chem Commun (Camb). 2022 Aug 11;58(65):9116-9119
pubmed: 35880535
Life Sci. 2020 Dec 1;262:118562
pubmed: 33038378
Biochim Biophys Acta Proteins Proteom. 2019 Oct;1867(10):890-908
pubmed: 30853581
Methods Enzymol. 1994;244:266-84
pubmed: 7845214
J Phys Org Chem. 2018 Aug;31(8):
pubmed: 30983696
Chem Commun (Camb). 2020 Mar 24;56(24):3567-3570
pubmed: 32104795
Acta Neuropathol Commun. 2021 Nov 24;9(1):188
pubmed: 34819159
J Mol Biol. 2008 Mar 14;377(1):148-61
pubmed: 18234215
Bioconjug Chem. 2008 Mar;19(3):588-91
pubmed: 18275131
J Neurochem. 2016 May;137(3):331-59
pubmed: 26852372
Nat Protoc. 2011 Mar;6(3):365-87
pubmed: 21372817
ACS Chem Biol. 2020 May 15;15(5):1127-1133
pubmed: 31999086
J Mol Biol. 1993 May 5;231(1):29-40
pubmed: 8496964
J Biol Chem. 2017 Aug 11;292(32):13482-13497
pubmed: 28611062
Nat Struct Mol Biol. 2016 May;23(5):409-15
pubmed: 27018801
Curr Opin Chem Biol. 2015 Oct;28:123-30
pubmed: 26253346
Chem Rev. 2019 Dec 11;119(23):11819-11856
pubmed: 31675223
Curr Opin Chem Biol. 2008 Feb;12(1):60-5
pubmed: 18282482
Genome Res. 1999 May;9(5):492-8
pubmed: 10330129
J Am Chem Soc. 2015 Feb 11;137(5):1734-7
pubmed: 25625321
PLoS Genet. 2018 Jun 1;14(6):e1007405
pubmed: 29856734
Chem Commun (Camb). 2018 Jan 18;54(7):833-836
pubmed: 29313531
Chem Commun (Camb). 2017 Oct 5;53(80):11072-11075
pubmed: 28948265
J Am Chem Soc. 2013 Dec 18;135(50):18806-14
pubmed: 24303933
Nature. 2008 May 22;453(7194):489-4
pubmed: 18497818
Mol Neurodegener. 2021 Dec 18;16(1):83
pubmed: 34922583
Phys Chem Chem Phys. 2012 Oct 5;14(37):12671-86
pubmed: 22806312
Nature. 2018 May;557(7706):558-563
pubmed: 29743672
J Mol Biol. 2003 Oct 17;333(2):345-54
pubmed: 14529621
Sci Rep. 2021 Sep 15;11(1):18406
pubmed: 34526629
J Phys Chem B. 2020 Nov 25;124(47):10653-10662
pubmed: 33196192
Nucleic Acids Res. 2006 May 09;34(8):2463-71
pubmed: 16684994
Proc Natl Acad Sci U S A. 2023 Jan 10;120(2):e2217493120
pubmed: 36598938
Org Biomol Chem. 2017 May 3;15(17):3603-3610
pubmed: 28397914
Proc Natl Acad Sci U S A. 1987 Jun;84(12):3987-91
pubmed: 3108885
Acta Neuropathol. 2010 Aug;120(2):131-43
pubmed: 20563819
Environ Mol Mutagen. 2019 May;60(4):368-384
pubmed: 30447030
Chem Sci. 2020 Sep 10;11(47):12746-12754
pubmed: 33889379
J Mol Biol. 2022 Dec 15;434(23):167859
pubmed: 36270580
Methods Mol Biol. 2004;261:161-6
pubmed: 15064456
Front Microbiol. 2020 Aug 04;11:1785
pubmed: 32849403
Chemphyschem. 2005 May;6(5):858-70
pubmed: 15884068
Proc Natl Acad Sci U S A. 1967 Aug;58(2):719-26
pubmed: 5233469
ACS Chem Biol. 2018 Oct 19;13(10):2855-2861
pubmed: 30216041
Biochemistry. 1986 Nov 4;25(22):6866-75
pubmed: 2948553
Nucleic Acids Res. 2011 Aug;39(15):6546-57
pubmed: 21576225
J Med Chem. 2003 Sep 25;46(20):4259-64
pubmed: 13678404
Angew Chem Int Ed Engl. 2013 Jun 10;52(24):6210-3
pubmed: 23629972
PLoS Genet. 2011 Sep;7(9):e1002244
pubmed: 21912525
Protein Sci. 2023 May;32(5):e4633
pubmed: 36974585
Sci Rep. 2019 Feb 27;9(1):2937
pubmed: 30814575