Heart-Type Fatty Acid Binding Protein Binds Long-Chain Acylcarnitines and Protects against Lipotoxicity.
FABP3
fatty acids
heart-type fatty acid-binding protein
isothermal titration calorimetry
long-chain acylcarnitines
nuclear magnetic resonance
palmitate
palmitoylcarnitine
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
14 Mar 2023
14 Mar 2023
Historique:
received:
21
02
2023
revised:
10
03
2023
accepted:
11
03
2023
medline:
30
3
2023
entrez:
29
3
2023
pubmed:
30
3
2023
Statut:
epublish
Résumé
Heart-type fatty-acid binding protein (FABP3) is an essential cytosolic lipid transport protein found in cardiomyocytes. FABP3 binds fatty acids (FAs) reversibly and with high affinity. Acylcarnitines (ACs) are an esterified form of FAs that play an important role in cellular energy metabolism. However, an increased concentration of ACs can exert detrimental effects on cardiac mitochondria and lead to severe cardiac damage. In the present study, we evaluated the ability of FABP3 to bind long-chain ACs (LCACs) and protect cells from their harmful effects. We characterized the novel binding mechanism between FABP3 and LCACs by a cytotoxicity assay, nuclear magnetic resonance, and isothermal titration calorimetry. Our data demonstrate that FABP3 is capable of binding both FAs and LCACs as well as decreasing the cytotoxicity of LCACs. Our findings reveal that LCACs and FAs compete for the binding site of FABP3. Thus, the protective mechanism of FABP3 is found to be concentration dependent.
Identifiants
pubmed: 36982599
pii: ijms24065528
doi: 10.3390/ijms24065528
pmc: PMC10058761
pii:
doi:
Substances chimiques
acylcarnitine
0
Fatty Acid Binding Protein 3
0
Fatty Acid-Binding Proteins
0
Fatty Acids
0
Carnitine
S7UI8SM58A
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Union's Horizon 2020 research and innovation program project FAT4BRAIN
ID : 857394
Organisme : Latvian Institute of Organic Synthesis
ID : IG-2023-04
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