Enzymatic Aminoacylation of tRNA
Aminoacylation
Arginylation
tRNA
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
5
4
2023
entrez:
3
4
2023
pubmed:
4
4
2023
Statut:
ppublish
Résumé
This chapter describes the preparation of pre-charged Arg-tRNA that can be used in arginylation reaction. While in a typical arginylation reaction arginyl-tRNA synthetase (RARS) is normally included as a component of the reaction and continually charges tRNA during arginylation, it is sometimes necessary to separate the charging and the arginylation step, in order to perform each reaction under controlled conditions, e.g., for measuring the kinetics or determining the effect of different compounds and chemicals on the reaction. In such cases, tRNA
Identifiants
pubmed: 37010755
doi: 10.1007/978-1-0716-2942-0_14
doi:
Substances chimiques
Arginine-tRNA Ligase
EC 6.1.1.19
RNA, Transfer, Arg
0
RNA, Transfer
9014-25-9
Amino Acyl-tRNA Synthetases
EC 6.1.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107-111Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM122505
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS102435
Pays : United States
Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Wang J, Han X, Saha, S, Xu, T, Rai, R, Zhang, F, Wolf, YI, Wolfson A, Yates JRR, III, 2 Kashina A (2011) Arginyltransferase is an ATP-Independent self-regulating enzyme that forms distinct functional complexes In Vivo. Chem Biol 2011 Jan 28; 18(1):121–130. https://doi.org/10.1016/j.chembiol.2010.10.016
Avcilar-Kucukgoze I, Gamper H, Polte C, Ignatova Z, Kraetzner R, Shtutman M, Hou YM, Dong DW, Kashina A (2020) tRNA(Arg)-derived fragments can serve as arginine donors for protein arginylation. Cell Chem Biol 27(7):839–849. e834. https://doi.org/10.1016/j.chembiol.2020.05.013
doi: 10.1016/j.chembiol.2020.05.013
pubmed: 32553119
pmcid: 7409373
Peacock JR, Walvoord RR, Chang AY, Kozlowski MC, Gamper H, Hou YM (2014) Amino acid-dependent stability of the acyl linkage in aminoacyl-tRNA. RNA 20(6):758–764. https://doi.org/10.1261/rna.044123.113
doi: 10.1261/rna.044123.113
pubmed: 24751649
pmcid: 4024630