Papaya latex mediated synthesis of prism shaped proteolytic gold nanozymes.

Latex Carica / physiology Gold Peptide Hydrolases Cysteine Proteases Vegetables

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
12 04 2023
Historique:
received: 25 07 2022
accepted: 27 03 2023
medline: 14 4 2023
entrez: 12 4 2023
pubmed: 13 4 2023
Statut: epublish

Résumé

Beyond natural enzymes, the artificially synthesized nanozymes have attracted a significant interest as it can overcome the limitations of the former. Here, we report synthesis of shape controlled nanozymes showing proteolytic activity using Carica papaya L. (papaya) latex. The nanozymes synthesized under optimized reaction conditions exhibited sharp SPR peak around 550 nm with high abundance (45.85%) of prism shaped particles. FTIR analysis and coagulation test indicated the presence of papaya latex enzymes as capping agents over the gold nanoprisms. The milk clot assay and the inhibition test with egg white confirmed the proteolytic activity of the nanozymes and the presence of cysteine protease on it, respectively. The nanozymes were found to be biocompatible and did not elicit any toxic response in both in-vitro and in-vivo study. Based on our findings, we envisage that these biocompatible, shape-specific nanozymes can have potential theragnostic applications.

Identifiants

DOI: 10.1038/s41598-023-32409-7 PMID: 37045854 PMC: PMC10097869
pubmed: 37045854
doi: 10.1038/s41598-023-32409-7
pii: 10.1038/s41598-023-32409-7
pmc: PMC10097869
doi:

Substances chimiques

Latex 0
Gold 7440-57-5
Peptide Hydrolases EC 3.4.-
Cysteine Proteases EC 3.4.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

5965

Informations de copyright

© 2023. The Author(s).

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Auteurs

Ajoy Kumar Das (AK)

Department of Botany, Arya Vidyapeeth College, Gopinath Nagar, Guwahati, Assam, 781016, India. ajoykrdasavc@gmail.com.
Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam, India. ajoykrdasavc@gmail.com.

Jon Jyoti Kalita (JJ)

Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam, India.

Maina Borah (M)

Department of Botany, Pandu College, Pandu, Guwahati, Assam, 781012, India.

Suradip Das (S)

Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam, India.

Manav Sharma (M)

Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam, India.

Dhiren Saharia (D)

Saharia's Path Lab and Blood Bank, Guwahati, Assam, 781 005, India.

Kushal Konwar Sarma (KK)

Department of Surgery and Radiology, College of Veterinary Sciences, Assam Agriculture University Campus, Khanapara, Guwahati, Assam, 781 022, India.

Samrat Bora (S)

Department of Botany, Arya Vidyapeeth College, Gopinath Nagar, Guwahati, Assam, 781016, India.

Utpal Bora (U)

Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam, India.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Latex › Papaya latex mediated synthesis of prism...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Caricaceae › Carica › Papaya latex mediated synthesis of prism...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Or › Papaya latex mediated synthesis of prism...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Papaya latex mediated synthesis of prism...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Peptide hydrolases › Cysteine proteases › Papaya latex mediated synthesis of prism...
questionsmedicales.fr › Aliments et boissons › Aliments › Légumes › Papaya latex mediated synthesis of prism...