Ligand-targeted fishing of α-glucosidase inhibitors from Tribulus terrestris L. based on chitosan-functionalized multi-walled carbon nanotubes with immobilized α-glucosidase.
Chitosan-functionalized multi-walled carbon nanotubes
IMS
Immobilization
UHPLC-QTOF-MS
α-Glucosidase inhibitors
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
received:
17
01
2023
accepted:
21
03
2023
revised:
21
02
2023
medline:
17
5
2023
pubmed:
15
4
2023
entrez:
14
4
2023
Statut:
ppublish
Résumé
α-Glucosidase inhibitors in natural products are one of the promising drugs for the treatment of type 2 diabetes. However, due to the complexity of the matrix, it is challenging to comprehensibly clarify the specific pharmacodynamic substances. In this study, a novel high-throughput inhibitor screening strategy was established based on covalent binding of α-glucosidase on chitosan-functionalized multi-walled carbon nanotubes coupled with high-resolution mass spectrometry. The synthesized MWCNTs@CS@GA@α-Glu was characterized by TEM, SEM, FTIR, Raman, and TG. Performance studies showed that the microreactor exhibited stronger thermostability and pH tolerance than that of the free one while maintaining its inherent catalytic activity. Feasibility study applying a model mixture of known α-glucosidase ligand and non-ligands indicated the selectivity and specificity of the system. By integrating ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-QTOF-MS) with ion mobility mass spectrometry (IMS), 15 ligands were obtained and tentatively identified from Tribulus terrestris L., including 8 steroidal saponins, 4 flavonoids, and 3 alkaloids. These inhibitors were further validated by in vivo experiments and molecular docking simulation.
Identifiants
pubmed: 37058167
doi: 10.1007/s00216-023-04666-y
pii: 10.1007/s00216-023-04666-y
doi:
Substances chimiques
alpha-Glucosidases
EC 3.2.1.20
Chitosan
9012-76-4
Glycoside Hydrolase Inhibitors
0
Nanotubes, Carbon
0
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2677-2692Subventions
Organisme : National Natural Science Foundation of China
ID : 82173952
Organisme : Science and the Youth Innovation Promotion Association of CAS
ID : 2019227
Informations de copyright
© 2023. Springer-Verlag GmbH Germany, part of Springer Nature.
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