Electrochemical biomimetic enzyme cascade amplification combined with target-induced DNA walker for detection of thrombin.
Amperometry
Biomimetic enzyme cascade reaction
Electrochemical biosensor
Nanozyme
Signal amplification
Thrombin detection
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
20 04 2023
20 04 2023
Historique:
received:
16
11
2022
accepted:
29
03
2023
medline:
24
4
2023
pubmed:
20
4
2023
entrez:
20
04
2023
Statut:
epublish
Résumé
Fe-N-doped carbon nanomaterials (Fe-N/CMs) were designed as a novel biomimetic enzyme with excellent peroxidase-like activity to achieve high-efficient enzyme cascade catalytic amplification with the aid of glucose oxidase (GOx), which was further combined with target-induced DNA walker amplification to develop a sensitive electrochemical biosensor for thrombin detection. Impressively, massive output DNA was transformed from small amounts of target thrombin by highly effective DNA walker amplification as protein-converting strategy, which could then induce the immobilization of functionalized nanozyme on the electrode surface to achieve the high-efficient electrochemical biomimetic enzyme cascade amplification. As a result, an amplified enzyme cascade catalytic signal was measured for thrombin detection ranging from 0.01 pM to 1 nM with a low detection limit of 3 fM. Importantly, the new biomimetic enzyme cascade reaction coupled the advantages of natural enzyme and nanozyme, which paved an avenue to construct varied artificial multienzymes amplification systems for biosensing, bioanalysis, and disease diagnosis applications.
Identifiants
pubmed: 37079080
doi: 10.1007/s00604-023-05769-0
pii: 10.1007/s00604-023-05769-0
doi:
Substances chimiques
Thrombin
EC 3.4.21.5
DNA
9007-49-2
Glucose Oxidase
EC 1.1.3.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
188Subventions
Organisme : National Natural Science Foundation of China
ID : 81873606
Organisme : National Natural Science Foundation of China
ID : 22174113
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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