Combining on-line spectroscopy with synchrotron and X-ray free electron laser crystallography.

Synchrotrons Electrons Crystallography, X-Ray Spectrum Analysis Lasers

Journal

Current opinion in structural biology
ISSN: 1879-033X
Titre abrégé: Curr Opin Struct Biol
Pays: England
ID NLM: 9107784

Informations de publication

Date de publication:
06 2023
Historique:
received: 23 01 2023
revised: 22 03 2023
accepted: 05 04 2023
medline: 5 6 2023
pubmed: 7 5 2023
entrez: 6 5 2023
Statut: ppublish

Résumé

With the recent advances in serial crystallography methods at both synchrotron and X-ray free electron laser sources, more details of intermediate or transient states of the catalytic reactions are being revealed structurally. These structural studies of reaction dynamics drive the need for on-line in crystallo spectroscopy methods to complement the crystallography experiment. The recent applications of combined spectroscopy and crystallography methods enable on-line determination of in crystallo reaction kinetics and structures of catalytic intermediates, sample integrity, and radiation-induced sample modifications, if any, as well as heterogeneity of crystals from different preparations or sample batches. This review describes different modes of spectroscopy that are combined with the crystallography experiment at both synchrotron and X-ray free-electron laser facilities, and the complementary information that each method can provide to facilitate the structural study of enzyme catalysis and protein dynamics.

Identifiants

DOI: 10.1016/j.sbi.2023.102604 PMID: 37148654
pubmed: 37148654
pii: S0959-440X(23)00078-7
doi: 10.1016/j.sbi.2023.102604
pii:
doi:

Types de publication

Journal Article Review Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

102604

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM055302
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM110501
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM126289
Pays : United States

Informations de copyright

Copyright © 2023 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest statement Nothing declared.

Auteurs

Hiroki Makita (H)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Philipp S Simon (PS)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Jan Kern (J)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: JFKern@lbl.gov.

Junko Yano (J)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: JYano@lbl.gov.

Vittal K Yachandra (VK)

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: VKYachandra@lbl.gov.

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Classifications MeSH

questionsmedicales.fr › Équipement et fournitures › Équipement et fournitures pour les applications des radiations › Accélérateurs de particules › Synchrotrons › Combining on-line spectroscopy with...
questionsmedicales.fr › Phénomènes physiques › Phénomènes magnétiques › Phénomènes électromagnétiques › Électrons › Combining on-line spectroscopy with...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › Combining on-line spectroscopy with...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Combining on-line spectroscopy with...
questionsmedicales.fr › Équipement et fournitures › Équipement et fournitures pour les applications des radiations › Lasers › Combining on-line spectroscopy with...