Structure of the actively translating plant 80S ribosome at 2.2 Å resolution.
Journal
Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677
Informations de publication
Date de publication:
06 2023
06 2023
Historique:
received:
21
07
2022
accepted:
29
03
2023
medline:
22
6
2023
pubmed:
9
5
2023
entrez:
8
5
2023
Statut:
ppublish
Résumé
In plant cells, translation occurs in three compartments: the cytosol, the plastids and the mitochondria. While the structures of the (prokaryotic-type) ribosomes in plastids and mitochondria are well characterized, high-resolution structures of the eukaryotic 80S ribosomes in the cytosol have been lacking. Here the structure of translating tobacco (Nicotiana tabacum) 80S ribosomes was solved by cryo-electron microscopy with a global resolution of 2.2 Å. The ribosome structure includes two tRNAs, decoded mRNA and the nascent peptide chain, thus providing insights into the molecular underpinnings of the cytosolic translation process in plants. The map displays conserved and plant-specific rRNA modifications and the positions of numerous ionic cofactors, and it uncovers the role of monovalent ions in the decoding centre. The model of the plant 80S ribosome enables broad phylogenetic comparisons that reveal commonalities and differences in the ribosomes of plants and those of other eukaryotes, thus putting our knowledge about eukaryotic translation on a firmer footing.
Identifiants
pubmed: 37156858
doi: 10.1038/s41477-023-01407-y
pii: 10.1038/s41477-023-01407-y
pmc: PMC10281867
doi:
Substances chimiques
RNA, Ribosomal
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
987-1000Subventions
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : 416210002
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : 221545957
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : 394046635
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : 421152132
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : ZO 302/5-1
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : SFB-TRR 175 (A4)
Organisme : EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)
ID : 956314
Informations de copyright
© 2023. The Author(s).
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