A novel strategy on the study of whole intestinal metabolic profiles for Polygalae Radix before and after processing.
UNIFI-pathway mode
aromatic carboxylic acids
isotope-labelling derivatisation
processed Polygalae Radix
whole intestinal bacteria metabolism
Journal
Phytochemical analysis : PCA
ISSN: 1099-1565
Titre abrégé: Phytochem Anal
Pays: England
ID NLM: 9200492
Informations de publication
Date de publication:
Jul 2023
Jul 2023
Historique:
revised:
18
04
2023
received:
17
02
2023
accepted:
21
04
2023
medline:
12
7
2023
pubmed:
12
5
2023
entrez:
11
5
2023
Statut:
ppublish
Résumé
Relieving toxicity and enhancing a calming effect after processing Polygalae Radix (PR) are widely known. Aromatic carboxylic acids (ACAs) may be crucial processed products. However, due to the limited detection methods for ACAs, the whole metabolic profiles via intestinal bacteria are still not very clear. Designing a novel strategy for the detection of ACAs and tracking the whole metabolic profiles before and after processing PR. The stable-isotope labelling derivatisation (SILD) method based on multidimensional ultra-high performance liquid chromatography coupled with a mass spectrometer (UHPLC-MS) technology and UNIFI-pathway mode was firstly designed to systematically study the metabolisms of all the drug-derived ingredients ranging from m/z 100 to 2000 in processing PR via intestinal bacteria. Firstly, the SILD with UHPLC coupled with a triple-quadrupole MS technology was designed to trace eight ACA metabolites of the processed PR with intestinal bacteria. Additionally, the UHPLC coupled with a quadrupole time-of-flight MS with UNIFI-pathway mode was adopted to monitor relatively big metabolites. The metabolism mechanism of ACAs (eight kinds) and the relatively big molecular metabolites (98 kinds) were deeply traced in PR, PR with refined honey (HP), and PR with licorice (LP) via the intestinal bacteria. Totally 106 intact metabolic profiles of drug-derived ingredients were presented. Importantly, the influence of LP on the metabolism of compounds after incubation of intestinal bacteria was greater than that of HP. This research provides a comprehensive and systematic guidance for further study on in vivo metabolisms of the processed PR.
Substances chimiques
Drugs, Chinese Herbal
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
540-547Subventions
Organisme : National Natural Science Foundation of China
ID : 82073973
Organisme : National Natural Science Foundation of China
ID : 82204604
Organisme : Natural Science Foundation of Hebei Province
ID : H2022209012
Organisme : Science and the Youth Innovation Promotion Association of CAS
ID : 2019227
Informations de copyright
© 2023 John Wiley & Sons Ltd.
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