Improved salt-tolerance of transgenic soybean by stable over-expression of AhBADH gene from Atriplex hortensis.
AhBADH gene
Atriplex hortensis
Salt-tolerance
Transgenic soybean
Journal
Plant cell reports
ISSN: 1432-203X
Titre abrégé: Plant Cell Rep
Pays: Germany
ID NLM: 9880970
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
received:
31
03
2023
accepted:
04
05
2023
revised:
29
04
2023
medline:
17
7
2023
pubmed:
17
5
2023
entrez:
17
5
2023
Statut:
ppublish
Résumé
The salt-tolerance of transgenic soybean cleared for environmental release was improved by stable over-expression of AhBADH gene from Atriplex hortensis, which was demonstrated through molecular analysis and field experiments. An effective strategy for increasing the productivity of major crops under salt stress conditions is the development of transgenics that harbor genes responsible for salinity tolerance. Betaine aldehyde dehydrogenase (BADH) is a key enzyme involved in the biosynthesis of the osmoprotectant, glycine betaine (GB), and osmotic balance in plants, and several plants transformed with BADH gene have shown significant improvements in salt tolerance. However, very few field-tested transgenic cultivars have been reported, as most of the transgenic studies are limited to laboratory or green house experiments. In this study, we demonstrated through field experiments that AhBADH from Atriplex hortensis confers salt tolerance when transformed into soybean (Glycine max L.). AhBADH was successfully introduced into soybean by Agrobacterium mediated transformation. A total of 256 transgenic plants were obtained, out of which 47 lines showed significant enhancement of salt tolerance compared to non-transgenic control plants. Molecular analyses of the transgenic line TL2 and TL7 with the highest salt tolerance exhibited stable inheritance and expression of AhBADH in progenies with a single copy insertion. TL1, TL2 and TL7 exhibited stable enhanced salt tolerance and improved agronomic traits when subjected to 300mM NaCl treatment. Currently, the transgenic line TL2 and TL7 with stable enhanced salt tolerance, which have been cleared for environmental release, are under biosafety assessment. TL 2 and TL7 stably expressing AhBADH could then be applied in commercial breeding experiments to genetically improve salt tolerance in soybean.
Identifiants
pubmed: 37195504
doi: 10.1007/s00299-023-03031-8
pii: 10.1007/s00299-023-03031-8
doi:
Substances chimiques
Betaine-Aldehyde Dehydrogenase
EC 1.2.1.8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1291-1310Subventions
Organisme : China National Transgenic Organisms Breeding Project
ID : 2016ZX08004002-009
Organisme : China National Transgenic Organisms Breeding Project
ID : 2014ZX0800403B-002
Organisme : Jilin Provincial Science & Technology Project
ID : 20130206005NY
Organisme : Jilin Provincial Science & Technology Project
ID : 20210202002NC
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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