UMSBP2 is chromatin remodeler that functions in regulation of gene expression and suppression of antigenic variation in trypanosomes.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
23 06 2023
23 06 2023
Historique:
accepted:
03
05
2023
received:
16
12
2021
medline:
26
6
2023
pubmed:
19
5
2023
entrez:
19
5
2023
Statut:
ppublish
Résumé
Universal Minicircle Sequence binding proteins (UMSBPs) are CCHC-type zinc-finger proteins that bind the single-stranded G-rich UMS sequence, conserved at the replication origins of minicircles in the kinetoplast DNA, the mitochondrial genome of kinetoplastids. Trypanosoma brucei UMSBP2 has been recently shown to colocalize with telomeres and to play an essential role in chromosome end protection. Here we report that TbUMSBP2 decondenses in vitro DNA molecules, which were condensed by core histones H2B, H4 or linker histone H1. DNA decondensation is mediated via protein-protein interactions between TbUMSBP2 and these histones, independently of its previously described DNA binding activity. Silencing of the TbUMSBP2 gene resulted in a significant decrease in the disassembly of nucleosomes in T. brucei chromatin, a phenotype that could be reverted, by supplementing the knockdown cells with TbUMSBP2. Transcriptome analysis revealed that silencing of TbUMSBP2 affects the expression of multiple genes in T. brucei, with a most significant effect on the upregulation of the subtelomeric variant surface glycoproteins (VSG) genes, which mediate the antigenic variation in African trypanosomes. These observations suggest that UMSBP2 is a chromatin remodeling protein that functions in the regulation of gene expression and plays a role in the control of antigenic variation in T. brucei.
Identifiants
pubmed: 37207337
pii: 7173837
doi: 10.1093/nar/gkad402
pmc: PMC10287944
doi:
Substances chimiques
Chromatin
0
Histones
0
Variant Surface Glycoproteins, Trypanosoma
0
Protozoan Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5678-5698Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
Nucleic Acids Res. 2021 Apr 6;49(6):3242-3262
pubmed: 33660774
Antimicrob Agents Chemother. 2006 Apr;50(4):1195-201
pubmed: 16569829
Int J Parasitol. 1994 Sep;24(6):809-19
pubmed: 7982743
J Cell Biol. 2001 May 14;153(4):725-34
pubmed: 11352934
Nucleic Acids Res. 2013 Sep;41(16):7673-82
pubmed: 23804762
Annu Rev Microbiol. 2012;66:473-91
pubmed: 22994497
PLoS Pathog. 2020 Jan 2;16(1):e1008167
pubmed: 31895930
J Cell Biol. 1994 Aug;126(3):591-601
pubmed: 8045925
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Bioessays. 2018 Dec;40(12):e1800181
pubmed: 30370931
J Biol Chem. 1995 Sep 8;270(36):21339-45
pubmed: 7545668
Mol Microbiol. 2014 Nov;94(4):945-62
pubmed: 25266856
Mol Cell Biol. 1999 Oct;19(10):6940-52
pubmed: 10490631
J Biol Chem. 2006 Dec 8;281(49):37468-76
pubmed: 17046830
Cell Biosci. 2016 Feb 17;6:13
pubmed: 26889377
Science. 1975 Apr 11;188(4184):165-6
pubmed: 1090006
Nucleic Acids Res. 2019 Jan 25;47(2):634-647
pubmed: 30407533
Nucleic Acids Res. 2014 Nov 10;42(20):12899-911
pubmed: 25313155
J Mol Biol. 2020 Jul 10;432(15):4167-4185
pubmed: 31682833
Nucleic Acids Res. 2009 Jan;37(1):279-88
pubmed: 19039000
Curr Opin Struct Biol. 2017 Dec;47:9-16
pubmed: 28419835
Antioxid Redox Signal. 2010 Nov 1;13(9):1429-76
pubmed: 20446770
Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20725-20735
pubmed: 31554700
Q Rev Biophys. 2017 Jan;50:e5
pubmed: 29233217
Nucleic Acids Res. 2012 Nov 1;40(20):10150-60
pubmed: 22941664
Parasitology. 1993 Sep;107 ( Pt 3):237-47
pubmed: 8233587
Mol Microbiol. 2004 Jul;53(2):365-72
pubmed: 15228519
Genome Biol. 2014;15(12):550
pubmed: 25516281
Planta. 2013 Mar;237(3):653-64
pubmed: 23212213
Eukaryot Cell. 2008 Jan;7(1):86-101
pubmed: 17981991
Elife. 2021 Mar 30;10:
pubmed: 33783358
Nucleic Acids Res. 2018 Sep 6;46(15):7757-7771
pubmed: 30007364
Expert Rev Mol Med. 2012 May 29;14:e13
pubmed: 22640744
J Comput Biol. 2017 Nov;24(11):1138-1143
pubmed: 28715235
Eukaryot Cell. 2004 Apr;3(2):277-87
pubmed: 15075258
Curr Genomics. 2018 Feb;19(2):119-132
pubmed: 29491740
Trends Genet. 2006 Jun;22(6):320-9
pubmed: 16631276
Eukaryot Cell. 2012 Dec;11(12):1573-81
pubmed: 23104567
Sci Rep. 2021 Jan 15;11(1):1501
pubmed: 33452335
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3428-32
pubmed: 1103148
PLoS Pathog. 2012;8(8):e1002900
pubmed: 22952449
Nucleic Acids Res. 1998 Oct 15;26(20):4626-34
pubmed: 9753730
Open Biol. 2019 Nov 29;9(11):190182
pubmed: 31718509
Mol Biochem Parasitol. 2014 Jul;195(2):123-9
pubmed: 24859277
Methods Cell Biol. 2014;122:165-91
pubmed: 24857730
Mol Cell Biol. 1993 Dec;13(12):7766-73
pubmed: 8246992
Pathogens. 2021 Jul 30;10(8):
pubmed: 34451431
J Biol Chem. 1999 May 7;274(19):13419-26
pubmed: 10224106
FASEB J. 2001 Jan;15(1):34-42
pubmed: 11149891
Annu Rev Biochem. 2014;83:487-517
pubmed: 24905786
EMBO J. 2007 Nov 28;26(23):4856-66
pubmed: 17972917
Cell. 2020 Jan 9;180(1):150-164.e15
pubmed: 31883795
J Biol Chem. 2008 Nov 14;283(46):32085-98
pubmed: 18768469
Trends Cell Biol. 2007 Dec;17(12):586-92
pubmed: 17981466
Int J Mol Sci. 2021 Mar 31;22(7):
pubmed: 33807484
Nat Struct Mol Biol. 2007 Nov;14(11):997-1007
pubmed: 17984962
Nat Methods. 2013 Dec;10(12):1213-8
pubmed: 24097267
Biophys J. 2013 May 21;104(10):2222-34
pubmed: 23708362
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
J Biol Chem. 2008 Nov 14;283(46):32034-44
pubmed: 18799461
Curr Mol Med. 2004 Sep;4(6):623-47
pubmed: 15357213
Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1786-9
pubmed: 8446592
J Proteome Res. 2021 Jan 1;20(1):27-37
pubmed: 32962352
Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6891-5
pubmed: 1323120
J Cell Sci. 1999 Dec;112 ( Pt 24):4641-50
pubmed: 10574712
Curr Genet. 2005 Nov;48(5):277-99
pubmed: 16215758
Nat Biotechnol. 2011 Jan;29(1):24-6
pubmed: 21221095
Cell Microbiol. 2013 Dec;15(12):1984-93
pubmed: 24047558
EMBO J. 2016 Jul 1;35(13):1465-82
pubmed: 27225933
Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19250-5
pubmed: 18048338
FEBS Lett. 1999 Dec 17;463(3):285-8
pubmed: 10606739
Chromosoma. 2016 Mar;125(1):95-110
pubmed: 26223534
Nat Cell Biol. 2015 Aug;17(8):1014-23
pubmed: 26075356
Cell. 2017 May 18;169(5):780-791
pubmed: 28525751
Eur J Biochem. 2000 Feb;267(4):1030-8
pubmed: 10672011
Genome Biol. 2013 Apr 25;14(4):R36
pubmed: 23618408
Science. 2015 Mar 27;347(6229):1470-3
pubmed: 25814582
J Biol Chem. 2011 Nov 25;286(47):40566-74
pubmed: 21984849
Cell. 2006 Nov 3;127(3):495-508
pubmed: 17081973
Int J Parasitol. 2018 Aug;48(9-10):691-700
pubmed: 29778329
Eukaryot Cell. 2014 Jul;13(7):919-32
pubmed: 24879122
Bioinformatics. 2010 Mar 15;26(6):841-2
pubmed: 20110278
Genes Dev. 1999 Nov 1;13(21):2875-88
pubmed: 10557214
Chromosoma. 2000 Mar;108(8):501-13
pubmed: 10794572
PLoS Pathog. 2012;8(11):e1003010
pubmed: 23133390
mBio. 2021 Aug 31;12(4):e0172521
pubmed: 34311578
Eukaryot Cell. 2010 Jan;9(1):136-47
pubmed: 19915073
Mol Cell Biol. 1996 Feb;16(2):564-76
pubmed: 8552084
Trends Parasitol. 2015 Aug;31(8):346-9
pubmed: 25999027
Nat Rev Microbiol. 2010 Mar;8(3):185-95
pubmed: 20140026