The pGinger Family of Expression Plasmids.

Plasmids / genetics Promoter Regions, Genetic Escherichia coli / genetics Metabolic Engineering
molecular genetics plasmid synthetic biology

Journal

Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614

Informations de publication

Date de publication:
15 06 2023
Historique:
medline: 19 6 2023
pubmed: 22 5 2023
entrez: 22 5 2023
Statut: ppublish

Résumé

The pGinger suite of expression plasmids comprises 43 plasmids that will enable precise constitutive and inducible gene expression in a wide range of Gram-negative bacterial species. Constitutive vectors are composed of 16 synthetic constitutive promoters upstream of red fluorescent protein (RFP), with a broad-host-range BBR1 origin and a kanamycin resistance marker. The family also has seven inducible systems (Jungle Express, Psal/NahR, Pm/XylS, Prha/RhaS, LacO1/LacI, LacUV5/LacI, and Ptet/TetR) controlling RFP expression on BBR1/kanamycin plasmid backbones. For four of these inducible systems (Jungle Express, Psal/NahR, LacO1/LacI, and Ptet/TetR), we created variants that utilize the RK2 origin and spectinomycin or gentamicin selection. Relevant RFP expression and growth data have been collected in the model bacterium Escherichia coli as well as Pseudomonas putida. All pGinger vectors are available via the Joint BioEnergy Institute (JBEI) Public Registry.

Identifiants

DOI: 10.1128/spectrum.00373-23 PMID: 37212656 PMC: PMC10269703
pubmed: 37212656
doi: 10.1128/spectrum.00373-23
pmc: PMC10269703
doi:

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0037323

Déclaration de conflit d'intérêts

The authors declare a conflict of interest. J.D.K. has financial interests in Amyris, Ansa Biotechnologies, Apertor Pharma, Berkeley Yeast, Demetrix, Lygos, Napigen, ResVita Bio, and Zero Acre Farms.

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Auteurs

Allison N Pearson (AN)

Joint BioEnergy Institute, Emeryville, California, USA.
Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Department of Plant and Microbial Biology, University of California, Berkeley, California, USA.

Mitchell G Thompson (MG)

Joint BioEnergy Institute, Emeryville, California, USA.
Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Liam D Kirkpatrick (LD)

Joint BioEnergy Institute, Emeryville, California, USA.
Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Cindy Ho (C)

Joint BioEnergy Institute, Emeryville, California, USA.
Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Khanh M Vuu (KM)

Joint BioEnergy Institute, Emeryville, California, USA.
Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Lucas M Waldburger (LM)

Joint BioEnergy Institute, Emeryville, California, USA.
Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Department of Bioengineering, University of California, Berkeley, California, USA.

Jay D Keasling (JD)

Joint BioEnergy Institute, Emeryville, California, USA.
Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Department of Chemical and Biomolecular Engineering, University of California, Berkeley, California, USA.
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark.
Center for Synthetic Biochemistry, Institute for Synthetic Biology, Shenzhen Institutes for Advanced Technologies, Shenzhen, China.

Patrick M Shih (PM)

Joint BioEnergy Institute, Emeryville, California, USA.
Department of Plant and Microbial Biology, University of California, Berkeley, California, USA.
Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Innovative Genomics Institute, University of California, Berkeley, California, USA.
ORCID: 0000-0002-2119-3345

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › The pGinger Family of Expression Plasmids.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Éléments de régulation transcriptionnelle › Régions promotrices (génétique) › The pGinger Family of Expression Plasmids.
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › The pGinger Family of Expression Plasmids.
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Génie métabolique › The pGinger Family of Expression Plasmids.