PhosY-secretome profiling combined with kinase-substrate interaction screening defines active c-Src-driven extracellular signaling.

Protein-Tyrosine Kinases / metabolism Secretome Signal Transduction Phosphotransferases Phosphorylation src Homology Domains Cell Communication src-Family Kinases
CP: Cancer CP: Molecular biology PxxP motif Src tyrosine kinase TIMP2 blocking antibodies phosphoproteomic post-translational modification secretome tissue inhibitors of metalloproteinases tumor growth inhibition

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
27 06 2023
Historique:
received: 03 10 2022
revised: 07 04 2023
accepted: 03 05 2023
medline: 4 10 2023
pubmed: 27 5 2023
entrez: 27 5 2023
Statut: ppublish

Résumé

c-Src tyrosine kinase is a renowned key intracellular signaling molecule and a potential target for cancer therapy. Secreted c-Src is a recent observation, but how it contributes to extracellular phosphorylation remains elusive. Using a series of domain deletion mutants, we show that the N-proximal region of c-Src is essential for its secretion. The tissue inhibitor of metalloproteinases 2 (TIMP2) is an extracellular substrate of c-Src. Limited proteolysis-coupled mass spectrometry and mutagenesis studies verify that the Src homology 3 (SH3) domain of c-Src and the P

Identifiants

DOI: 10.1016/j.celrep.2023.112539 PMID: 37243593 PMC: PMC10569185
pubmed: 37243593
pii: S2211-1247(23)00550-8
doi: 10.1016/j.celrep.2023.112539
pmc: PMC10569185
mid: NIHMS1912762
pii:
doi:

Substances chimiques

Protein-Tyrosine Kinases EC 2.7.10.1
Phosphotransferases EC 2.7.-
src-Family Kinases EC 2.7.10.2

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

112539

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM139932
Pays : United States

Informations de copyright

Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Références

Cell. 1994 Feb 11;76(3):411-3
pubmed: 8313462
Nat Rev Cancer. 2017 Jan;17(1):38-53
pubmed: 27932800
Cell. 2010 Dec 23;143(7):1174-89
pubmed: 21183079
Dev Cell. 2004 Dec;7(6):855-69
pubmed: 15572128
Carcinogenesis. 2022 Jun 4;43(5):405-418
pubmed: 35436325
Science. 1999 Jan 29;283(5402):655-61
pubmed: 9924018
Biochem Biophys Res Commun. 2004 Nov 26;324(4):1155-64
pubmed: 15504335
Mol Clin Oncol. 2020 Oct;13(4):21
pubmed: 32765869
Cancer Cell. 2019 Mar 18;35(3):347-367
pubmed: 30889378
Structure. 2005 Jun;13(6):861-71
pubmed: 15939018
Annu Rev Biochem. 2000;69:373-98
pubmed: 10966463
Proteomics. 2013 May;13(10-11):1672-86
pubmed: 23585443
J Biol Chem. 2000 Apr 14;275(15):11312-9
pubmed: 10753943
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
Nucleic Acids Res. 2015 Jan;43(Database issue):D512-20
pubmed: 25514926
Cell. 1994 Mar 11;76(5):933-45
pubmed: 7510218
Cancer Sci. 2022 Apr;113(4):1168-1181
pubmed: 35043517
EMBO J. 1999 May 4;18(9):2459-71
pubmed: 10228160
Biochim Biophys Acta Mol Cell Res. 2017 Nov;1864(11 Pt A):1989-2000
pubmed: 28578911
Biomedicines. 2022 Feb 09;10(2):
pubmed: 35203617
Exp Cell Res. 2007 Jul 15;313(12):2651-66
pubmed: 17537435
Proc Natl Acad Sci U S A. 2015 Jun 23;112(25):E3189-98
pubmed: 26056257
Oncogene. 2004 Oct 18;23(48):7918-27
pubmed: 15489910
Trends Biochem Sci. 1995 Nov;20(11):470-5
pubmed: 8578591
Semin Cancer Biol. 2010 Jun;20(3):161-8
pubmed: 20470890
Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1488-93
pubmed: 17242355
Cell. 2011 Nov 23;147(5):992-1009
pubmed: 22118458
Nat Rev Cancer. 2016 Feb;16(2):83-98
pubmed: 26822576
Mol Cell Proteomics. 2012 May;11(5):187-201
pubmed: 22322096
PLoS One. 2013 Aug 05;8(8):e70811
pubmed: 23940647
iScience. 2019 Feb 22;12:194-203
pubmed: 30690395
Sci Rep. 2019 Dec 27;9(1):20142
pubmed: 31882975
J Biol Chem. 2019 Aug 30;294(35):13186-13197
pubmed: 31331936
Sci Signal. 2016 Mar 22;9(420):re3
pubmed: 27016527
Cell Rep. 2022 Jul 12;40(2):111039
pubmed: 35830801
Cell Rep. 2019 Aug 13;28(7):1894-1906.e6
pubmed: 31412254
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):12253-7
pubmed: 7527558
Br J Cancer. 2021 Jan;124(1):102-114
pubmed: 33204027
Trends Cell Biol. 2008 Jul;18(7):322-9
pubmed: 18515107
iScience. 2018 Mar 23;1:87-96
pubmed: 30227959
Proc Natl Acad Sci U S A. 2017 Mar 21;114(12):3175-3180
pubmed: 28270605
Genes Dev. 1996 Aug 1;10(15):1845-57
pubmed: 8756343
J Proteome Res. 2020 Jul 2;19(7):2563-2574
pubmed: 32396726
Commun Biol. 2019 Sep 3;2:325
pubmed: 31508500
J Cell Biochem. 2017 Jan;118(1):66-73
pubmed: 27232975
Mol Cell Biol. 1990 Mar;10(3):1000-9
pubmed: 1689455
F1000Res. 2019 Oct 18;8:1774
pubmed: 31819800
Biochem Soc Trans. 2016 Aug 15;44(4):1026-34
pubmed: 27528748
Cells. 2022 Jun 29;11(13):
pubmed: 35805153
Biochemistry. 1998 Feb 24;37(8):2145-59
pubmed: 9485361
Sci Rep. 2013;3:1295
pubmed: 23416516
Front Mol Biosci. 2021 Jul 28;8:729513
pubmed: 34395534
Sci Rep. 2019 Mar 1;9(1):3265
pubmed: 30824759
Science. 1986 Mar 21;231(4744):1431-4
pubmed: 2420005
Mol Cell Proteomics. 2013 Feb;12(2):343-55
pubmed: 23161513
Cancer Cell. 2004 Sep;6(3):209-14
pubmed: 15380511
J Mol Biol. 2016 Feb 22;428(4):720-725
pubmed: 26410586
Cell. 2014 Aug 28;158(5):1033-1044
pubmed: 25171405
Nat Rev Cancer. 2010 Mar;10(3):165-80
pubmed: 20179713
J Cell Biol. 1992 Jul;118(2):321-33
pubmed: 1378446
Colloids Surf B Biointerfaces. 2016 Feb 1;138:17-25
pubmed: 26638178
Front Cell Dev Biol. 2020 Nov 30;8:569377
pubmed: 33330449
FEBS Lett. 2012 Aug 14;586(17):2609-14
pubmed: 22710157
Clin Exp Metastasis. 2009;26(4):273-87
pubmed: 18498004
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
Nat Protoc. 2017 Nov;12(11):2391-2410
pubmed: 29072706
Biochim Biophys Acta. 1996 Jun 7;1287(2-3):121-49
pubmed: 8672527
Nat Rev Cancer. 2010 Jan;10(1):9-22
pubmed: 20029421
Proc Natl Acad Sci U S A. 2017 Nov 21;114(47):12495-12500
pubmed: 29109268
Cell Commun Signal. 2021 Jun 30;19(1):67
pubmed: 34193161
Biochem Biophys Res Commun. 2020 Feb 18;:
pubmed: 32085898
Bioinformatics. 2010 Apr 1;26(7):966-8
pubmed: 20147306
Sci Signal. 2008 Jul 08;1(27):re6
pubmed: 18612141
Nature. 1997 Feb 13;385(6617):595-602
pubmed: 9024657
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W429-32
pubmed: 17483518
J Am Soc Nephrol. 2009 Feb;20(2):363-79
pubmed: 19056867
J Biol Chem. 2000 Aug 25;275(34):26416-22
pubmed: 10827176
Biochim Biophys Acta. 1996 Dec 12;1314(3):191-225
pubmed: 8982275
Nucleic Acids Res. 2021 Jan 8;49(D1):D480-D489
pubmed: 33237286
J Biol Chem. 2000 Aug 25;275(34):26411-5
pubmed: 10827175
Nat Rev Cancer. 2004 Jun;4(6):470-80
pubmed: 15170449
Cell. 2015 Jun 18;161(7):1619-32
pubmed: 26091039
Protein Sci. 2020 Feb;29(2):350-359
pubmed: 31697410
Carcinogenesis. 2020 May 14;41(3):313-325
pubmed: 31621840
Biochemistry. 2017 Dec 12;56(49):6423-6433
pubmed: 29140689
Nature. 2016 Sep 14;537(7620):347-55
pubmed: 27629641
Cell. 2003 Jul 25;114(2):171-80
pubmed: 12887919
Mol Cell Proteomics. 2014 Apr;13(4):1034-49
pubmed: 24505115
EMBO J. 1997 Dec 15;16(24):7261-71
pubmed: 9405355
J Mol Biol. 1998 Dec 11;284(4):1133-40
pubmed: 9837731
Mol Med Rep. 2020 Oct;22(4):2941-2947
pubmed: 32945400
Crit Rev Biochem Mol Biol. 2018 Oct;53(5):535-563
pubmed: 30183386
EMBO J. 1994 Oct 17;13(20):4745-56
pubmed: 7525268
Biophys Rev. 2013 Mar;5(1):29-39
pubmed: 28510178
Nat Rev Clin Oncol. 2009 Oct;6(10):587-95
pubmed: 19787002
Sci Adv. 2019 Nov 20;5(11):eaax8849
pubmed: 31799396

Auteurs

Sarah J Backe (SJ)

Department of Urology, SUNY Upstate Medical University, Syracuse, NY 13210, USA; Upstate Cancer Center, SUNY Upstate Medical University, Syracuse, NY 13210, USA.

SarahBeth D Votra (SD)

Department of Urology, SUNY Upstate Medical University, Syracuse, NY 13210, USA; Upstate Cancer Center, SUNY Upstate Medical University, Syracuse, NY 13210, USA.

Matthew P Stokes (MP)

Cell Signaling Technology, Inc., Danvers, MA 01923, USA.

Endre Sebestyén (E)

The Bioinformatics CRO, Inc., Orlando, FL, USA.

Matteo Castelli (M)

Dipartimento di Chimica, Università di Pavia, 27100 Pavia, Italy.

Luca Torielli (L)

Dipartimento di Chimica, Università di Pavia, 27100 Pavia, Italy.

Giorgio Colombo (G)

Dipartimento di Chimica, Università di Pavia, 27100 Pavia, Italy.

Mark R Woodford (MR)

Department of Urology, SUNY Upstate Medical University, Syracuse, NY 13210, USA; Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA; Upstate Cancer Center, SUNY Upstate Medical University, Syracuse, NY 13210, USA.

Mehdi Mollapour (M)

Department of Urology, SUNY Upstate Medical University, Syracuse, NY 13210, USA; Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA; Upstate Cancer Center, SUNY Upstate Medical University, Syracuse, NY 13210, USA.

Dimitra Bourboulia (D)

Department of Urology, SUNY Upstate Medical University, Syracuse, NY 13210, USA; Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA; Upstate Cancer Center, SUNY Upstate Medical University, Syracuse, NY 13210, USA. Electronic address: bourmpod@upstate.edu.

Articles similaires

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

The FGF/FGFR/c-Myc axis as a promising therapeutic target in multiple myeloma.

Arianna Giacomini, Sara Taranto, Giorgia Gazzaroli et al.
1.00
Humans Multiple Myeloma Receptors, Fibroblast Growth Factor Fibroblast Growth Factors Proto-Oncogene Proteins c-myc

Exploring transcriptomic mechanisms underlying pulmonary adaptation to diverse environments in Indian rams.

Ritika Gera, Reena Arora, Pooja Chhabra et al.
1.00
Animals Lung India Sheep Transcriptome

Calcineurin inhibition enhances

Priyanka Das, Alejandro Aballay, Jogender Singh
1.00
Animals Caenorhabditis elegans Longevity Caenorhabditis elegans Proteins Calcineurin

Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-tyrosine kinases › PhosY-secretome profiling combined with...
questionsmedicales.fr › Métabolisme › Métabolome › Sécrétome › PhosY-secretome profiling combined with...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › PhosY-secretome profiling combined with...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › PhosY-secretome profiling combined with...
questionsmedicales.fr › Métabolisme › Phosphorylation › PhosY-secretome profiling combined with...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Motifs et domaines d'intéraction protéique › Domaine d'homologie SRC › PhosY-secretome profiling combined with...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Communication cellulaire › PhosY-secretome profiling combined with...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-tyrosine kinases › src-Family kinases › PhosY-secretome profiling combined with...