Appearance of ghost bands in gel electrophoresis depending on agarose concentration.
CD33
CYP1A1
DNA splicing variant
artefactual band
ghost band
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
revised:
19
05
2023
received:
04
11
2022
accepted:
20
05
2023
medline:
17
8
2023
pubmed:
1
6
2023
entrez:
1
6
2023
Statut:
ppublish
Résumé
Standard agarose gel electrophoresis is a widely used method to analyse diversity of nucleic acids. Certain conditions, however, may give rise to artefactual bands. We report on artefactual bands frequently occurring, especially when partially homologous nucleic acids, such as splicing variants of DNA transcripts, are analysed simultaneously. Interestingly, to some extent agarose concentration may influence the occurrence of artefactual bands.
Identifiants
pubmed: 37259607
doi: 10.1002/elps.202300041
doi:
Substances chimiques
Sepharose
9012-36-6
DNA
9007-49-2
Nucleic Acids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1206-1209Informations de copyright
© 2023 The Authors. Electrophoresis published by Wiley-VCH GmbH.
Références
Raj T, Ryan KJ, Replogle JM, Chibnik LB, Rosenkrantz L, Tang A, et al. CD33: increased inclusion of exon 2 implicates the Ig V-set domain in Alzheimer's disease susceptibility. Hum Mol Genet. 2014;23(10):2729-36.
Green M, Sambrook J. Alkaline agarose gel electrophoresis. Cold Spring Harb Protoc. 2021;11. https://doi.org/10.1101/pdb.prot100438
Leung YK, Lau KM, Mobley J, Jiang Z, Ho SM. Overexpression of cytochrome P450 1A1 and its novel spliced variant in ovarian cancer cells: alternative subcellular enzyme compartmentation may contribute to carcinogenesis. Cancer Res. 2005;65(9):3726-34.