Fluorometric Determination of DNA Nanostructure Biostability.
DNA nanostructures
DNA nanotechnology
DNA origami
biostability
nuclease degradation
Journal
ACS applied bio materials
ISSN: 2576-6422
Titre abrégé: ACS Appl Bio Mater
Pays: United States
ID NLM: 101729147
Informations de publication
Date de publication:
21 08 2023
21 08 2023
Historique:
medline:
22
8
2023
pubmed:
1
6
2023
entrez:
1
6
2023
Statut:
ppublish
Résumé
The analysis and improvement of DNA nanostructure biostability is one of the keys areas of progress needed in DNA nanotechnology applications. Here, we present a plate-compatible fluorometric assay for measuring DNA nanostructure biostability using the common intercalator ethidium bromide. We demonstrate the assay by testing the biostability of duplex DNA, a double crossover DNA motif, and a DNA origami nanostructure against different nucleases and in fetal bovine serum. This method scales well to measure a large number of samples using a plate reader and can complement existing methods for assessing and developing robust DNA nanostructures.
Identifiants
pubmed: 37262927
doi: 10.1021/acsabm.3c00287
doi:
Substances chimiques
DNA
9007-49-2
Deoxyribonucleases
EC 3.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
3074-3078Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM124720
Pays : United States
Organisme : NIA NIH HHS
ID : R03 AG076599
Pays : United States