Folding Double-Stranded DNA into Designed Shapes with Triplex-Forming Oligonucleotides.
DNA nanotechnology
Hoogsteen
origami
self-assembly
triplex
Journal
Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
revised:
07
06
2023
received:
17
03
2023
medline:
5
10
2023
pubmed:
14
6
2023
entrez:
13
6
2023
Statut:
ppublish
Résumé
The compaction and organization of genomic DNA is a central mechanism in eukaryotic cells, but engineered architectural control over double-stranded DNA (dsDNA) is notably challenging. Here, long dsDNA templates are folded into designed shapes via triplex-mediated self-assembly. Triplex-forming oligonucleotides (TFOs) bind purines in dsDNA via normal or reverse Hoogsteen interactions. In the triplex origami methodology, these non-canonical interactions are programmed to compact dsDNA (linear or plasmid) into well-defined objects, which demonstrate a variety of structural features: hollow and raster-filled, single- and multi-layered, with custom curvatures and geometries, and featuring lattice-free, square-, or honeycomb-pleated internal arrangements. Surprisingly, the length of integrated and free-standing dsDNA loops can be modulated with near-perfect efficiency; from hundreds down to only six bp (2 nm). The inherent rigidity of dsDNA promotes structural robustness and non-periodic structures of almost 25.000 nt are therefore formed with fewer unique starting materials, compared to other DNA-based self-assembly methods. Densely triplexed structures also resist degradation by DNase I. Triplex-mediated dsDNA folding is methodologically straightforward and orthogonal to Watson-Crick-based methods. Moreover, it enables unprecedented spatial control over dsDNA templates.
Identifiants
pubmed: 37311656
doi: 10.1002/adma.202302497
doi:
Substances chimiques
Oligonucleotides
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2302497Subventions
Organisme : Novo Nordic Foundation
ID : NNF17OC0028070
Organisme : Marie Skłodowska-Curie ITN project DNA-Robotics
ID : 765703
Organisme : Marie Skłodowska-Curie ITN project CONSENSE
ID : 955623
Informations de copyright
© 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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