Dissecting the Arginine and Lysine Biosynthetic Pathways and Their Relationship in Haloarchaeon Natrinema gari J7-2 via Endogenous CRISPR-Cas System-Based Genome Editing.


Journal

Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614

Informations de publication

Date de publication:
17 08 2023
Historique:
medline: 21 8 2023
pubmed: 22 6 2023
entrez: 22 6 2023
Statut: ppublish

Résumé

The evolutionary relationship between arginine and lysine biosynthetic pathways has been well established in bacteria and hyperthermophilic archaea but remains largely unknown in haloarchaea. Here, the endogenous CRISPR-Cas system was harnessed to edit arginine and lysine biosynthesis-related genes in the haloarchaeon Natrinema gari J7-2. The Δ

Identifiants

pubmed: 37347159
doi: 10.1128/spectrum.00288-23
pmc: PMC10433800
doi:

Substances chimiques

Lysine K3Z4F929H6
Arginine 94ZLA3W45F
Amino Acids 0
Glutamates 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0028823

Déclaration de conflit d'intérêts

The authors declare no conflict of interest.

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Auteurs

Yi Wu (Y)

Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China.

Jia Zhang (J)

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.

Bingxue Wang (B)

Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China.

Yanyan Zhang (Y)

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.

Huai Li (H)

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.

Yang Liu (Y)

Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China.

Jing Yin (J)

Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China.

Dan He (D)

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.

Hongyi Luo (H)

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.

Fei Gan (F)

Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China.
Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Wuhan, China.

Bing Tang (B)

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Wuhan, China.

Xiao-Feng Tang (XF)

Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China.
Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Wuhan, China.

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Classifications MeSH