Triplet-Encoded Prebiotic RNA Aminoacylation.
Journal
Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056
Informations de publication
Date de publication:
26 07 2023
26 07 2023
Historique:
medline:
27
7
2023
pubmed:
12
7
2023
entrez:
12
7
2023
Statut:
ppublish
Résumé
The encoding step of translation involves attachment of amino acids to cognate tRNAs by aminoacyl-tRNA synthetases, themselves the product of coded peptide synthesis. So, the question arises─before these enzymes evolved, how were primordial tRNAs selectively aminoacylated? Here, we demonstrate enzyme-free, sequence-dependent, chemoselective aminoacylation of RNA. We investigated two potentially prebiotic routes to aminoacyl-tRNA acceptor stem-overhang mimics and analyzed those oligonucleotides undergoing the most efficient aminoacylation. Overhang sequences do not significantly influence the chemoselectivity of aminoacylation by either route. For aminoacyl-transfer from a mixed anhydride donor strand, the chemoselectivity and stereoselectivity of aminoacylation depend on the terminal three base pairs of the stem. The results support early suggestions of a second genetic code in the acceptor stem.
Identifiants
pubmed: 37435826
doi: 10.1021/jacs.3c03931
pmc: PMC10375532
doi:
Substances chimiques
RNA
63231-63-0
RNA, Transfer
9014-25-9
Amino Acyl-tRNA Synthetases
EC 6.1.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15971-15980Subventions
Organisme : Medical Research Council
ID : MC_UP_A024_1009
Pays : United Kingdom
Références
Int J Mol Sci. 2022 Dec 12;23(24):
pubmed: 36555394
IUBMB Life. 2019 Aug;71(8):1088-1098
pubmed: 31190358
J Am Chem Soc. 2022 Aug 3;144(30):13920-13927
pubmed: 35880790
RNA. 2020 Feb;26(2):199-208
pubmed: 31719125
Proc Natl Acad Sci U S A. 2015 Dec 15;112(50):15396-401
pubmed: 26621738
Nucleic Acids Res. 2023 Feb 28;51(4):1528-1570
pubmed: 36744444
Nat Chem. 2015 Apr;7(4):301-7
pubmed: 25803468
Proc Natl Acad Sci U S A. 2010 May 4;107(18):8298-303
pubmed: 20385807
Nature. 1988 May 12;333(6169):117-8
pubmed: 3367984
Proc Natl Acad Sci U S A. 1999 May 11;96(10):5470-5
pubmed: 10318907
Science. 1968 Jul 26;161(3839):387
pubmed: 5661298
J Mol Evol. 2020 Aug;88(6):501-509
pubmed: 32382786
Nat Chem. 2020 Nov;12(11):1023-1028
pubmed: 33093680
J Theor Biol. 1992 Nov 21;159(2):199-214
pubmed: 1294846
Cell. 1995 Jun 30;81(7):983-6
pubmed: 7600584
Science. 2008 Oct 17;322(5900):404
pubmed: 18927386
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4334-8
pubmed: 279919
J Am Chem Soc. 1974 Aug 7;96(16):5283-4
pubmed: 4853687
Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6199-202
pubmed: 7687063
Science. 1988 May 6;240(4853):793-6
pubmed: 2452483
Science. 1994 Jan 14;263(5144):191-7
pubmed: 7506844
ACS Chem Biol. 2010 Jul 16;5(7):655-7
pubmed: 20499895
J Mol Evol. 1978 May 12;11(1):9-16
pubmed: 26807
Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):6729-34
pubmed: 8041690
Nature. 1988 May 12;333(6169):140-5
pubmed: 3285220
J Biol Chem. 2013 Sep 13;288(37):26856-63
pubmed: 23867455
J Am Chem Soc. 2009 Apr 15;131(14):5257-63
pubmed: 19351205
Nat Chem. 2021 Aug;13(8):751-757
pubmed: 34312504
J Mol Biol. 1968 Dec;38(3):367-79
pubmed: 4887876
RNA. 2020 Aug;26(8):910-936
pubmed: 32303649
Biosystems. 2022 Nov;221:104750
pubmed: 35970477
Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11467-71
pubmed: 7972085
J Am Chem Soc. 2011 Apr 20;133(15):6044-50
pubmed: 21438575
Nature. 1989 Feb 2;337(6206):478-81
pubmed: 2915692
FEBS Lett. 2021 Apr;595(7):913-924
pubmed: 33460451
Life (Basel). 2017 Mar 23;7(2):
pubmed: 28333103
Nature. 1965 Aug 7;207(997):597-9
pubmed: 5883631
J Mol Biol. 1966 Aug;19(2):548-55
pubmed: 5969078
Nature. 1966 Feb 26;209(5026):868-70
pubmed: 5922773
J Am Chem Soc. 2022 Mar 9;144(9):4254-4259
pubmed: 35230111
J Am Chem Soc. 2021 Aug 4;143(30):11836-11842
pubmed: 34283595
Nucleic Acids Res. 1998 Nov 15;26(22):5017-35
pubmed: 9801296
Cell. 2000 Jun 9;101(6):569-72
pubmed: 10892641