DNA-origami-directed virus capsid polymorphism.
Journal
Nature nanotechnology
ISSN: 1748-3395
Titre abrégé: Nat Nanotechnol
Pays: England
ID NLM: 101283273
Informations de publication
Date de publication:
10 2023
10 2023
Historique:
received:
25
10
2022
accepted:
06
06
2023
medline:
23
10
2023
pubmed:
18
7
2023
entrez:
17
7
2023
Statut:
ppublish
Résumé
Viral capsids can adopt various geometries, most iconically characterized by icosahedral or helical symmetries. Importantly, precise control over the size and shape of virus capsids would have advantages in the development of new vaccines and delivery systems. However, current tools to direct the assembly process in a programmable manner are exceedingly elusive. Here we introduce a modular approach by demonstrating DNA-origami-directed polymorphism of single-protein subunit capsids. We achieve control over the capsid shape, size and topology by employing user-defined DNA origami nanostructures as binding and assembly platforms, which are efficiently encapsulated within the capsid. Furthermore, the obtained viral capsid coatings can shield the encapsulated DNA origami from degradation. Our approach is, moreover, not limited to a single type of capsomers and can also be applied to RNA-DNA origami structures to pave way for next-generation cargo protection and targeting strategies.
Identifiants
pubmed: 37460794
doi: 10.1038/s41565-023-01443-x
pii: 10.1038/s41565-023-01443-x
pmc: PMC10575778
doi:
Substances chimiques
Capsid Proteins
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1205-1212Subventions
Organisme : European Research Council
ID : 101002258
Pays : International
Organisme : European Research Council
ID : 85670
Pays : International
Informations de copyright
© 2023. The Author(s).
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