Enzymatic synthesis of branched chain fatty acid-enriched structured triacylglycerols via esterification with glycerol.

Triglycerides / chemistry Glycerol Esterification Molecular Docking Simulation Fatty Acids / metabolism
Branched-chain fatty acid-enriched structured triacylglycerol Enzymatic esterification Lanolin Molecular distillation Molecular docking

Journal

Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639

Informations de publication

Date de publication:
15 Dec 2023
Historique:
received: 22 05 2023
revised: 06 07 2023
accepted: 17 07 2023
medline: 24 8 2023
pubmed: 31 7 2023
entrez: 30 7 2023
Statut: ppublish

Résumé

While branched-chain fatty acids (BCFA)-enriched triacylglycerols (TAG) has various health benefits, its preparation has not been reported. This study aimed to synthesize high-purity BCFA-enriched structured TAG. First, BCFA was enriched from lanolin through saponification, calcification, and urea complexation. Next, BCFA-enriched TAG was synthesized by enzymatic esterification of BCFA and glycerol. Then, lipases were screened by molecular docking and practical experiments, which suggested that Lipozyme 435 was the best lipase for esterification since it had the lowest binding energy. Structured TAG containing 92.23% BCFA was synthesized under conditions optimized by single-factor experiments. Furthermore, molecular distillation was adapted to remove excess fatty acids and small molecule impurities. Finally, high-purity BCFA-enriched structured lipid containing 70.26% TAG was obtained. Overall, this study successfully developed a method for synthesizing BCFA-enriched structured TAG, which holds great promise for applications in value-added foods.

Identifiants

DOI: 10.1016/j.foodchem.2023.136943 PMID: 37517224
pubmed: 37517224
pii: S0308-8146(23)01561-3
doi: 10.1016/j.foodchem.2023.136943
pii:
doi:

Substances chimiques

Triglycerides 0
Glycerol PDC6A3C0OX
Fatty Acids 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

136943

Informations de copyright

Copyright © 2023 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Yaqi Huang (Y)

Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, National Engineering Research Center for Functional Food, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road Wuxi, Jiangsu 214122, PR China.

Houyue Li (H)

Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, National Engineering Research Center for Functional Food, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road Wuxi, Jiangsu 214122, PR China.

Zixin Wang (Z)

Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, National Engineering Research Center for Functional Food, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road Wuxi, Jiangsu 214122, PR China.

Yijie Fu (Y)

Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, National Engineering Research Center for Functional Food, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road Wuxi, Jiangsu 214122, PR China.

Yang Chen (Y)

Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, National Engineering Research Center for Functional Food, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road Wuxi, Jiangsu 214122, PR China.

Xiaosan Wang (X)

Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, National Engineering Research Center for Functional Food, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road Wuxi, Jiangsu 214122, PR China. Electronic address: xiaosanw@jiangnan.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Lipides › Glycérides › Triglycéride › Enzymatic synthesis of branched chain fatty...
questionsmedicales.fr › Glucides › Polyols › Dérivés du glycérol › Glycérol › Enzymatic synthesis of branched chain fatty...
questionsmedicales.fr › Métabolisme › Estérification › Enzymatic synthesis of branched chain fatty...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Simulation de docking moléculaire › Enzymatic synthesis of branched chain fatty...
questionsmedicales.fr › Lipides › Acides gras › Enzymatic synthesis of branched chain fatty...