Thermodynamic Characterization of Nucleic Acid Nanoparticles Hybridization by UV Melting.
DNA nanoparticles
Nucleic acid hybridization
Nucleic acid thermodynamics
RNA nanoparticles
UV melting
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
14
8
2023
pubmed:
13
8
2023
entrez:
12
8
2023
Statut:
ppublish
Résumé
The advances in nucleic acid nanotechnology have given rise to various elegantly designed structural complexes fabricated from DNA, RNA, chemically modified RNA strands, and their mixtures. The structural properties of NA nanoparticles (NANP) generally dictate and significantly impact biological function; and thus, it is critical to extract information regarding relative stabilities of the different structural forms. The adequate stability assessment requires knowledge of thermodynamic parameters that can be empirically derived using conventional UV-melting technique. The focus of this chapter is to describe methodology to evaluate thermodynamic data of NANPs complexation based on DNA 12 base-pair (bp) duplex formation as an example.
Identifiants
pubmed: 37572278
doi: 10.1007/978-1-0716-3417-2_9
doi:
Substances chimiques
DNA
9007-49-2
Nucleic Acids
0
RNA
63231-63-0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
151-161Subventions
Organisme : NIBIB NIH HHS
ID : R15 EB031388
Pays : United States
Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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