Epivolve: A Protocol for Site-Directed Antibodies.
Antibody
Biopanning
Epivolve
Phage display
Site-directed
Site-specific
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
pmc-release:
01
01
2024
medline:
11
9
2023
pubmed:
8
9
2023
entrez:
7
9
2023
Statut:
ppublish
Résumé
Researchers can often successfully generate antibodies to predicted epitopes. Especially when the epitopes are on the surface of a protein or in a hydrophilic loop. But it is difficult to direct recombinant antibodies to bind either to- or near a specific amino acid on a protein or peptide. We have developed a unique immune-targeting strategy, that we call "Epivolve," that enables us to make site-specific antibodies (Abs). Epivolve technology leverages a highly immunogenic modified amino acid that acts as a "pseudo-hapten" immuno-target and takes advantage of Ab affinity maturation technologies to make high-affinity site-specific antibodies. Epivolve functions by the evolution of an Ab paratope to either synonymous or especially non-synonymous amino acid (aa) binding. Here we describe the use of Epivolve technology in phage display and the protocols for developing site-specific antibodies.
Identifiants
pubmed: 37679640
doi: 10.1007/978-1-0716-3381-6_29
pmc: PMC10568616
mid: NIHMS1932761
doi:
Substances chimiques
Amino Acids
0
Antibodies
0
Epitopes
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
587-601Subventions
Organisme : NIGMS NIH HHS
ID : R43 GM146473
Pays : United States
Organisme : NIGMS NIH HHS
ID : R43 GM149009
Pays : United States
Organisme : NIAID NIH HHS
ID : R44 AI177126
Pays : United States
Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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