An improved digestion and analysis procedure for silicon in plant tissue.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2023
2023
Historique:
received:
10
04
2023
accepted:
12
07
2023
medline:
11
9
2023
pubmed:
8
9
2023
entrez:
8
9
2023
Statut:
epublish
Résumé
Silicon (Si) in plant tissues reduces abiotic and biotic stress, but it is incorporated as silica (SiO2), which is difficult to solubilize for analysis. We modified an oven-induced tissue-digestion and analysis method to improve Si solubilization and validated its accuracy by quantifying the mass-balance recovery of Si from the hydroponic solution and plant tissues of cucumber (Cucumis sativus). Leaf, stem, and root tissues were dried, finely-ground, and digested in 12.5 molar sodium hydroxide at 95°C for 4 hours. Solutions were then acidified with 6 molar hydrochloric acid to achieve a pH below 2 for measurement of Si using the molybdate blue colorimetric method. Interference of phosphorus in the analysis was minimized by increasing the addition of oxalic acid from 0.6 to 1.1 molar. We recovered 101% ± 13% of the expected Si, calculated using mass-balance recovery, in leaf, stem, and root tissues across 15 digestions. This Si recovery was fourteen-fold higher than the standard acid-extraction method and similar to a USDA-ARS alkaline-extraction method. Our procedure offers a low-cost, accurate method for extraction and analysis of Si in plant tissues.
Identifiants
pubmed: 37682894
doi: 10.1371/journal.pone.0289151
pii: PONE-D-23-10481
pmc: PMC10490927
doi:
Substances chimiques
Silicon
Z4152N8IUI
Silicon Dioxide
7631-86-9
Oxalic Acid
9E7R5L6H31
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0289151Informations de copyright
Copyright: © 2023 Langenfeld, Bugbee. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Plant Physiol. 1958 Sep;33(5):339-43
pubmed: 16655143
Plant Physiol. 2002 Dec;130(4):2111-7
pubmed: 12481095
Curr Opin Plant Biol. 2003 Jun;6(3):268-72
pubmed: 12753977
Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):11-7
pubmed: 11607449
FEMS Microbiol Lett. 2005 Aug 1;249(1):1-6
pubmed: 16006059
Cell Mol Life Sci. 2008 Oct;65(19):3049-57
pubmed: 18560761
Front Plant Sci. 2017 Mar 23;8:411
pubmed: 28386269