Development of a joint derivatization protocol for the unequivocal identification of the monosaccharide composition in four dendrobium polysaccharides and free monosaccharide by GC-MS.
GC-MS
acetylation
dendrobium polysaccharides
derivatization
monosaccharide composition
Journal
Biomedical chromatography : BMC
ISSN: 1099-0801
Titre abrégé: Biomed Chromatogr
Pays: England
ID NLM: 8610241
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
revised:
02
08
2023
received:
25
03
2023
accepted:
23
08
2023
medline:
23
11
2023
pubmed:
13
9
2023
entrez:
13
9
2023
Statut:
ppublish
Résumé
The determination of monosaccharides is crucial for studying the structure of polysaccharides and the composition of free monosaccharides in living organisms. Based on previous derivatization gas chromatography-mass spectrometry (GC-MS) methods, we aimed to develop a novel analytical protocol for better quantifying monosaccharides. In this study, sugar alcohol acetylation, saccharonitrile acetylation, silylation and a combination of sugar alcohols acetylation and saccharonitrile acetylation were compared. The optimal method was verified with the monosaccharide determination of four polysaccharides and four free monosaccharides from Dendrobium. The results showed that the novel combined derivatization method was superior to the other three methods in terms of content analysis of monosaccharides. Furthermore, it possessed good linearity (all calibration curves showed relative coefficients ≥ 0.999), sensitivity, precision (relative standard deviation < 2%), and accuracy (recovery, 95.7-105%). Finally, the novel method established in this study was successfully employed in determining the monosaccharide composition of four polysaccharides and four free monosaccharide samples from Dendrobium.
Substances chimiques
Monosaccharides
0
Polysaccharides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e5743Subventions
Organisme : Natural Science Foundation of Anhui Scientific Research and Innovation Team of Quality Evaluation and Improvement of Traditional Chinese Medicine
ID : 2022AH010090
Organisme : High-Level Talent Project of West Anhui University
ID : WGKQ2022024
Organisme : Provincial Level Nature Science Foundation of Anhui Education Department
ID : KJ2020A0632
Organisme : Provincial Level Nature Science Foundation of Anhui Education Department
ID : KJ2020A0634
Organisme : Provincial Level Nature Science Foundation of Anhui Education Department
ID : KJ2021A0957
Organisme : Provincial Level Nature Science Foundation of Anhui Education Department
ID : KJ2019A0626
Organisme : Provincial Level Nature Science Foundation of Anhui Education Department
ID : KJ2019A0617
Organisme : Provincial Level Nature Science Foundation of Anhui Education Department
ID : KJ2019A0628
Organisme : School Level Projects of West Anhui University
ID : WGKQ2021030
Organisme : Open Fund of Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources
ID : TCMRPSU-2022-06
Informations de copyright
© 2023 John Wiley & Sons Ltd.
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