Targeting AKT2 in MDA-MB-231 Cells by Pyrazole Hybrids: Structural, Biological and Molecular Docking Studies.


Journal

Chemistry & biodiversity
ISSN: 1612-1880
Titre abrégé: Chem Biodivers
Pays: Switzerland
ID NLM: 101197449

Informations de publication

Date de publication:
Nov 2023
Historique:
received: 01 06 2023
accepted: 11 09 2023
medline: 29 11 2023
pubmed: 13 9 2023
entrez: 13 9 2023
Statut: ppublish

Résumé

Pyrazolic hybrids appended with naphthalene, p-chlorobenzene, o-phenol and toluene have been synthesized using Claisen Schmidt condensation reaction of 1-benzyl-3,5-dimethyl-1H-pyrazole-4-carbaldehyde. All compounds were characterized by various spectroscopic techniques. Compound (E)-3-(1-benzyl-3,5-dimethyl-1H-pyrazol-4-yl)-1-(4-chlorophenyl)prop-2-en-1-one crystallizes in monoclinic crystal system with C2/c space group. These synthesized compounds were tested for cytotoxic activity and among these compounds 4b and 5a shows prominent cytotoxic activity against triple-negative breast cancer (TNBC) cells MDA-MB-231 with IC50 values 47.72 μM and 24.25 μM, respectively. Distinguishing morphological changes were noticed in MDA-MB-231 cells treated with pyrazole hybrids contributing to apoptosis action. To get more insight into cytotoxic activity, in silico molecular docking of these compounds were performed and the results suggested that (E)-3-(1-benzyl-3,5-dimethyl-1H-pyrazol-4-yl)-1-(p-tolyl)prop-2-en-1-one and 1-(1'-benzyl-5-(4-chlorophenyl)-3',5'-dimethyl-3,4-dihydro-1'H,2H-[3,4'-bipyrazol]-2-yl)ethan-1-one binds to the prominent domain of Akt2 indicating their potential ability as Akt2 inhibitor. Moreover, from in silico ADME studies clearly demonstrated that these compounds may be regarded as a drug candidate for sub-lingual absorption based on log p values (2.157-4.924). These compounds also show promising antitubercular activity. The overall results suggest that pyrazolic hybrids with substitution at less sterically hindered positions have appealing potent cytotoxic activity and antituberculosis activity due to which they may act as multidrug candidate.

Identifiants

pubmed: 37702285
doi: 10.1002/cbdv.202300799
doi:

Substances chimiques

Antineoplastic Agents 0
Pyrazoles 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202300799

Subventions

Organisme : Principal, R. B. Narayanrao Borawake College, Shrirampur and Research Center, Shri Chhatrapati Shivaji Mahavidyalaya, Shrigonda
Organisme : UGC, New Delhi, India
ID : 47-1124-14
Organisme : UGC, New Delhi, India
ID : ST-56/WRO

Informations de copyright

© 2023 Wiley-VHCA AG, Zurich, Switzerland.

Références

S. Fustero, M. Sánchez-Roselló, P. Barrio, A. Simón-Fuentes, Chem. Rev. 2011, 111, 6984-7034.
A. Ansari, A. Ali, M. Asif, Shamsuzzaman, New J. Chem. 2016, 41, 16-41.
M. H. Ahmed, M. A. El-Hashash, M. I. Marzouk, A. M. El-Naggar, J. Heterocycl. Chem. 2018, 56, 114-123.
R. Sribalan, G. Banuppriya, M. Kirubavathi, A. Jayachitra, V. Padmini, Bioorg. Med. Chem. Lett. 2016, 26, 5624-5630.
S. Ningaiah, U. K. Bhadraiah, S. D. Doddaramappa, S. Keshavamurthy, C. Javarasetty, Bioorg. Med. Chem. Lett. 2014, 24, 245-248.
R. C. Khunt, V. M. Khedkar, R. S. Chawda, N. A. Chauhan, A. R. Parikh, E. C. Coutinho, Bioorg. Med. Chem. Lett. 2012, 22, 666-678.
A. A. Bekhit, A. M. M. Hassan, H. A. Abd El Razik, M. M. M. El-Miligy, E. J. El-Agroudy, A. E.-D. A. Bekhit, Eur. J. Med. Chem. 2015, 94, 30-44.
M. K. Vekariya, R. H. Vekariya, K. D. Patel, N. P. Raval, P. U. Shah, D. P. Rajani, N. K. Shah, ChemistrySelect 2018, 3, 6998-7008.
A. T. Baviskar, U. C. Banerjee, M. Gupta, R. Singh, S. Kumar, M. K. Gupta, S. Kumar, S. K. Raut, M. Khullar, S. Singh, R. Kumar, Bioorg. Med. Chem. Lett. 2013, 21, 5782-5793.
A. Kamal, A. B. Shaik, N. Jain, C. Kishor, A. Nagabhushana, B. Supriya, G. Bharath Kumar, S. S. Chourasiya, Y. Suresh, R. K. Mishra, A. Addlagatta, Eur. J. Med. Chem. 2015, 92, 501-513.
X.-X. Tao, Y.-T. Duan, L.-W. Chen, D.-J. Tang, M.-R. Yang, P.-F. Wang, C. Xu, H.-L. Zhu, Bioorg. Med. Chem. Lett. 2016, 26, 677-683.
Q. Fu, P.-P. Cai, L. Cheng, L.-K. Zhong, C.-X. Tan, Z.-H. Shen, L Han, T.-M. Xub, X.-H. Liua, Pest Manag. Sci. 2020, 76, 868-879.
J. Sun, X.-H. Lv, H.-Y. Qiu, Y.-T. Wang, Q.-R. Du, D.-D. Li, Y.-H. Yang, H.-L. Zhu, Eur. J. Med. Chem. 2013, 68, 1-9.
S. M. M. Faudzi, S. W. Leong, F. A. Auwal, F. Abas, L. K. Wai, S. Ahmad, C. L. Tham, K. Shaari, N. H. Lajis, B. M. Yamin, Arch. Pharm. 2021, 354, 200061.
H. Wan, G. M. Schroeder, A. C. Hart, J. Inghrim, J. Grebinski, J. S. Tokarski, M. V. Lorenzi, D. You, T. Mcdevitt, B. Penhallow, R. Vuppugalla, Y. Zhang, X. Gu, R. Iyer, L. J. Lombardo, G. L. Trainor, S. Ruepp, J. Lippy, Y. Blat, J. S. Sack, ACS Med. Chem. Lett. 2015, 6, 850-855.
C.-H. Tu, W.-H. Lin, Y.-H. Peng, T. Hsu, J.-S. Wu, C.-Y. Chang, C.-T. Lu, P.-C. Lyu, C. Shih, W.-T. Jiaang, S.-Y. Wu, J. Med. Chem. 2016, 59, 3906-3919.
J. Bronson, M. Dhar, W. Ewing, N. Lonberg, Annu. Rep. Med. Chem. 2012, 47, 499-569.
A.-L. Hsu, T.-T. Ching, D.-S. Wang, X. Song, V. M. Rangnekar, C.-S. Chen, J. Biochem. 2000, 275, 11397-11403.
A. Ansari, A. Ali, M. Asif, Shamsuzzaman, New J. Chem. 2016, 41, 16-41.
M. F. Khan, M. M. Alam, G. Verma, W. Akhtar, M. Akhter, M. Shaquiquzzaman, Eur. J. Med. Chem. 2016, 120, 170-201.
K. Karrouchi, S. Radi, Y. Ramli, J. Taoufik, Y. Mabkhot, F. Al-aizari, M. Ansar, Molecules 2018, 23, 134.
S. S. Abd El-Karim, M. M. Anwar, N. A. Mohamed, T. Nasr, S. A. Elseginy, Bioorg. Chem. 2015, 63, 1-12.
A. M. Farag, A. S. Mayhoub, S. E. Barakat, A. H. Bayomi, Bioorg. Med. Chem. 2008, 16, 881-889.
A. S. Hassan, G. O. Moustafa, H. M. Awad, E. S. Nossier, M. F. Mady, ACS Omega 2021, 6, 12361-12374.
A. Kamal, V. Srinivasulu, V. L. Nayak, M. Sathish, N. Shankaraiah, C. Bagul, N. V. S. Reddy, N. Rangaraj, N. Nagesh, ChemMedChem 2014, 9, 2084-2098.
B. P. Bandgar, S. S. Gawande, R. G. Bodade, N. M. Gawande, C. N. Khobragade, Bioorg. Med. Chem. 2009, 17, 8168-8173.
F. Herencia, M. L. Ferrandiz, A. Ubeda, J. N. Dominguez, J. E. Charris, G. M. Lobo, M. J. Alcaraz, Bioorg. Med. Chem. Lett. 1998, 8, 1169-1174.
M. Xu, P. Wu, F. Shen, J. Ji, K. P. Rakesh, Bioorg. Chem. 2019, 91, 103133.
A. Modzelewska, C. Pettit, G. Achanta, N. E. Davidson, P. Huang, S. R. Khan, Bioorg. Med. Chem. 2006, 14, 3491-3495.
J. N. Domínguez, C. León, J. Rodrigues, N. Gamboa de Domínguez, J. Gut, P. J. Rosenthal, J. Med. Chem. 2005, 48, 3654-3658.
A. Rammohan, J. S. Reddy, G. Sravya, C. N. Rao, G. V. Zyryanov, Env. Chem. Lett. 2020, 18, 433-458.
G. Kumar, V. S. Krishna, D. Sriram, S. M. Jachak, Arch. Pharm. 2020, 353, 2000077.
L. Wattenberg, Chalcones, J. Cell. Biochem. 1995, 59, 162-168.
R. De Vincenzo, G. Scambia, P. Benedetti Panici, F. O. Ranelletti, G. Bonanno, A. Ercoli, F. Delle Monache, F. Ferrari, M. Piantelli, S. Mancuso, Anti-Cancer Drug Des. 1995, 10, 481-490.
N. Maciejewska, M. Olszewski, J. Jurasz, M. Serocki, M. Dzierzynska, K. Cekala, E. Wieczerzak, M. Baginski, Sci. Rep. 2022, 12, 3703.
B. A. Bhat, K. L. Dhar, S. C. Puri, A. K. Saxena, M. Shanmugavel, G. N. Qazi, Bioorg. Med. Chem. Lett. 2005, 15, 3177-3180.
A. F. Macarini, T. U. C. Sobrinho, G. W. Rizzi, R. Corrêa, Med. Chem. Res. 2019, 28, 1235-1245.
W. Akhtar, A. Marella, M. M. Alam, M. F. Khan, M. Akhtar, T. Anwer, F. Khan, Md. N. F. Azam, M. A. Rizvi, M. Shaquiquzzaman, Arch. Pharm. 2021, 354, 2000116.
C. Bagul, G. K. Rao, V. K. K. Makani, J. R. Tamboli, M. Pal-Bhadra, A. Kamal, MedChemComm 2017, 8, 1810-1816.
O. Seitz, Angew. Chem. 2003, 115, 5143-5144.
M. A. Kira, M. O. Abdel-Rahman, K. Z. Gadalla, Tet Lett. 1969, 10, 109-110.
W.-S. Loh, C. Quah, T. Chia, H.-K. Fun, M. Sapnakumari, B. Narayana, B. Sarojini, Molecules 2013, 18, 2386-2396.
S. Y. Shin, J. H. Kim, A. Baker, Y. Lim, Y. H. Lee, Mol. Cancer Res. 2010, 8, 507-519.
N. Rhodes, D. A. Heerding, D. R. Duckett, D. J. Eberwein, V. B. Knick, T. J. Lansing, R. T. McConnell, T. M. Gilmer, S.-Y. Zhang, K. Robell, J. A. Kahana, R. S. Geske, E. V. Kleymenova, A. E. Choudhry, Z. Lai, J. D. Leber, E. A. Minthorn, S. L. Strum, E. R. Wood, P. S. Huang, Cancer Res. 2008, 68, 2366-2374.
G. Kaur, M. P. Mahajan, M. K. Pandey, P. Singh, S. R. Ramisetti, A. K. Sharma, Eur. J. Med. Chem. 2014, 86, 211-218.
J. Jiao, A. Wang, M. Chen, M.-Q. Wang, C.-L. Yang, New J. Chem. 2019, 43, 6350-6360.
J. R. Testa, A. Bellacosa, PNAS 2001, 98, 10983-10985.
K. N. Nam, M.-S. Son, J.-H. Park, E. H. Lee, Neuropharmacology 2008, 55, 819-825.
D. A. Heerding, N. Rhodes, J. D. Leber, T. J. Clark, R. M. Keenan, L. V. Lafrance, M. Li, I. G. Safonov, D. T. Takata, J. W. Venslavsky, D. S. Yamashita, A. E. Choudhry, R. A. Copeland, Z. Lai, M. D. Schaber, P. J. Tummino, S. L. Strum, E. R. Wood, D. R. Duckett, D. Eberwein, J. Med. Chem. 2008, 51, 5663-5679.
A. Spencer, S.-S. Yoon, S. J. Harrison, S. R. Morris, D. A. Smith, R. A. Brigandi, J. Gauvin, R. Kumar, J. B. Opalinska, C. Chen, Blood 2014, 124, 2190-2195.
J. A. Joyce, J. W. Pollard, Nat. Rev. Cancer 2008, 9, 239-252.
S. Y. Shin, C. G. Kim, Y. J. Jung, Y. Lim, Y. H. Lee, Sci. Rep. 2016, 6, 34134.
M. Martínez-Cifuentes, B. Weiss-López, L. Santos, R. Araya-Maturana, Molecules 2014, 19, 9354-9368.
G. S. Jedhe, D. Paul, R. G. Gonnade, M. K. Santra, E. Hamel, T. L. Nguyen, G. J. Sanjayan, Bioorg. Med. Chem. Lett. 2013, 23, 4680-4684.
C. A. Lipinski, Adv. Drug Delivery Rev. 2016, 101, 34-41.
O. V. Dolomanov, L. J. Bourhis, R. J. Gildea, J. A. K. Howard, H. Puschmann, J. Appl. Crystallogr. 2009, 42, 339-341.
G. M. Sheldrick, Acta Crystallogr. Sect. A 2015, 71, s9.
C. F. Macrae, I. J. Bruno, J. A. Chisholm, P. R. Edgington, P. McCabe, E. Pidcock, L. Rodriguez-Monge, R. Taylor, J. van de Streek, P. A. Wood, J. Appl. Crystallogr. 2008, 41, 466-470.

Auteurs

Sanjay S Gaikwad (SS)

Department of Chemistry, MES, Abasaheb Garware College, Pune, Maharashtra, India-, 411004.

Snehal K Nimal (SK)

Department of Biotechnology, Savitribai Phule Pune University, Pune, Maharashtra, India-, 411007.

Rushikesh Pol (R)

Department of Microbiology, Savitribai Phule Pune University, Pune, Maharashtra, India-, 411007.

Datta Markad (D)

Department of Chemistry, University of Liverpool, Liverpool, L69 7ZD, United Kingdom.

Amardeep R Jadhao (AR)

Department of Chemistry, Late Pushpadevi Patil Arts and Science College, Risod, Dist., Washim, Maharashtra, India-, 444506.

Umesh Jadhav (U)

Department of Microbiology, Savitribai Phule Pune University, Pune, Maharashtra, India-, 411007.

Anup N Kate (AN)

Department of Chemistry, MES, Abasaheb Garware College, Pune, Maharashtra, India-, 411004.

Rajesh N Gacche (RN)

Department of Biotechnology, Savitribai Phule Pune University, Pune, Maharashtra, India-, 411007.

Limbraj R Patil (LR)

Department of Chemistry, Maharaja Jivajirao Shinde Arts, Science, Commerce, College, Shrigonda, Maharashtra, India-, 413701.

Rajeev C Chikate (RC)

Department of Chemistry, MES, Abasaheb Garware College, Pune, Maharashtra, India-, 411004.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs
T-Lymphocytes, Regulatory Lung Neoplasms Proto-Oncogene Proteins p21(ras) Animals Humans

Pathogenic mitochondrial DNA mutations inhibit melanoma metastasis.

Spencer D Shelton, Sara House, Luiza Martins Nascentes Melo et al.
1.00
DNA, Mitochondrial Humans Melanoma Mutation Neoplasm Metastasis
Animals Hemiptera Insect Proteins Phylogeny Insecticides

Classifications MeSH