Genome-wide identification of nitrate transporter 1/peptide transporter family (NPF) genes reveals that PaNPF5.5 enhances nitrate uptake in sweet cherry under high nitrate condition.

Nitrate Transporters Nitrates / metabolism Prunus avium / genetics Anion Transport Proteins / genetics Nitrogen / metabolism Plant Proteins / genetics Gene Expression Regulation, Plant
NPF Nitrate uptake Overexpression PaNPF5.5 Sweet cherry

Journal

Gene
ISSN: 1879-0038
Titre abrégé: Gene
Pays: Netherlands
ID NLM: 7706761

Informations de publication

Date de publication:
20 Dec 2023
Historique:
received: 17 07 2023
revised: 10 09 2023
accepted: 11 09 2023
medline: 2 10 2023
pubmed: 15 9 2023
entrez: 14 9 2023
Statut: ppublish

Résumé

NITRATE TRANSPORTER 1 (NRT1)/PEPTIDETRANSPORTER (PTR) family (NPF) plays a significant role in nitrate transport. However, little is known about the NPF genes in sweet cherry. In this study, a total of 60 PaNPF genes in sweet cherry were identified by bioinformatics, which were divided into 8 families. Transcriptomic analysis showed that most PaNPF genes responded to both low and high nitrate conditions, especially PaNPF5.5, which was highly up-regulated under high nitrate condition. Molecular analysis showed that PaNPF5.5 was a transporter localized to the cell membrane. Further functional studies found that PaNPF5.5 overexpression promoted the growth of sweet cherry rootstock Gisela 6 by accelerating the nitrogen absorption process under high nitrate environment. Taken together, we believe that PaNPF5.5 plays an important role in regulating the transport of nitrate at high nitrate conditions, and provides a promising method for improving nitrate absorption efficiency at nitrogen excess environment.

Identifiants

DOI: 10.1016/j.gene.2023.147797 PMID: 37708922
pubmed: 37708922
pii: S0378-1119(23)00638-8
doi: 10.1016/j.gene.2023.147797
pii:
doi:

Substances chimiques

Nitrate Transporters 0
peptide permease 97599-47-8
Nitrates 0
Anion Transport Proteins 0
Nitrogen N762921K75
Plant Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

147797

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Jingtao Wang (J)

School of Agriculture, Ludong University, Yantai 264025, China; College of Life Sciences, Ludong University, Yantai 264025, China.

Lei Wang (L)

College of Life Sciences, Ludong University, Yantai 264025, China.

Xu Zhang (X)

Yantai Academy of Agricultural Sciences, Yantai, Shandong 264025, China.

Songlin Li (S)

School of Agriculture, Ludong University, Yantai 264025, China.

Xiaohui Wang (X)

School of Agriculture, Ludong University, Yantai 264025, China.

Lina Yang (L)

Department of Genetics and Cell Biology, Basic Medical College, Qingdao University, Qingdao 266071, China.

Fanlin Wu (F)

School of Agriculture, Ludong University, Yantai 264025, China. Electronic address: wufanlin1990@126.com.

Hongyan Su (H)

College of Agriculture and Forestry Sciences, Linyi University, Linyi 276000, China. Electronic address: suhongyan66@126.com.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Transporteurs de nitrate › Genome-wide identification of nitrate...
questionsmedicales.fr › Composés chimiques organiques › Nitrates › Genome-wide identification of nitrate...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Rosanae › Rosales › Rosaceae › Prunus › Prunus avium › Genome-wide identification of nitrate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Pompes ioniques › Transporteurs d'anions › Genome-wide identification of nitrate...
questionsmedicales.fr › Produits chimiques inorganiques › Gaz › Azote › Genome-wide identification of nitrate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Genome-wide identification of nitrate...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › Genome-wide identification of nitrate...